Dynamic transcriptome and histomorphology analysis of developmental traits of hindlimb thigh muscle from Odorrana tormota and its adaptability to different life history stages

BMC Genomics

Jun He¹  Shuaitao Deng¹  Guangxuan Liu¹  Hailong Wu¹  Shikun Li¹  Yilin Shu¹  Huijuan Zhang¹ 

[1] Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, School of Ecology and Environment, Anhui Normal University, Wuhu, China;Provincial Key Laboratory of Biotic Environment and Ecological Safety in Anhui, Wuhu, China;

关键词: Development;    Adaptability;    Hindlimb thigh muscle;    Histomorphology;    Transcriptome;    Odorrana tormota;    Anura amphibians;   

DOI  :  10.1186/s12864-021-07677-0

来源: Springer

【 摘 要 】

BackgroundSystematic studies on the development and adaptation of hindlimb muscles in anura amphibians are rare. Here, we integrated analysis of transcriptome and histomorphological data for the hindlimb thigh muscle of Odorrana tormota (concave-eared torrent frog) at different developmental stages, to uncover the developmental traits of hindlimb thigh muscle from O. tormota and its adaptability to different life history stages.ResultsThe development of hindlimb thigh muscle from O. tormota has the following characteristics. Before metamorphosis, myogenous cells proliferate and differentiate into myotubes, and form 11 muscle groups at G41; Primary myofibers and secondary myofibers appeared during metamorphosis; 11 muscle groups differentiated continuously to form myofibers, accompanied by myofibers hypertrophy after metamorphosis; During the growth process of O. tormota from G42 to G46, there were differences between the sexes in the muscle groups that differentiate into muscle fibers, indicating that there was sexual dimorphism in the hindlimb thigh muscles of O. tormota at the metamorphosis stages. Some genes and pathways related to growth, development, and movement ability of O. tormota at different developmental stages were obtained. In addition, some pathways associated with adaptation to metamorphosis and hibernation also were enriched. Furthermore, integrated analysis of the number of myofibers and transcriptome data suggested that myofibers of specific muscle groups in the hindlimbs may be degraded through lysosome and ubiquitin pathways to transform into energy metabolism and other energy-related substances to meet the physiological needs of hibernation.ConclusionsThese results provide further understanding the hindlimb thigh muscle development pattern of frogs and their adaption to life history stages.

【 授权许可】

CC BY