Alteration of payload in extracellular vesicles by crosstalk with mesenchymal stem cells from different origin

Journal of Nanobiotechnology

Tae Hoon Kong¹  Jeong-Eun Park¹  Dong Jun Park²  Young Joon Seo³ 

[1] Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan- ro, 26426, Wonju, Gangwon-do, South Korea;Research Institute of Hearing Enhancement, 26426, Wonju, Gangwon-do, South Korea;Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan- ro, 26426, Wonju, Gangwon-do, South Korea;Research Institute of Hearing Enhancement, 26426, Wonju, Gangwon-do, South Korea;Department of Surgery, University of California San Diego, 212 Dickinson Street, MC 8236, 92103, San Diego, CA, USA;Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan- ro, 26426, Wonju, Gangwon-do, South Korea;Research Institute of Hearing Enhancement, 26426, Wonju, Gangwon-do, South Korea;School of Pharmacy and Biomedical Sciences, Curtin University, 6102, Bentley, WA, Australia;

关键词: Extracellular vesicles;    Mesenchymal stem cell;    Antiinflammation;    Amyloid-β A4 precursor protein-binding family A member 2;    miR-638;   

DOI  :  10.1186/s12951-021-00890-9

来源: Springer

【 摘 要 】

BackgroundThe application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads.ResultsHere, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively.ConclusionsIn conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine.Graphical abstract

【 授权许可】

CC BY