miR-129-5p inhibits clear cell renal cell carcinoma cell proliferation, migration and invasion by targeting SPN

Cancer Cell International

Lijuan Wang¹  Na Zhang¹  Yifei Liu¹  Bin Gao¹  Yubo Zhang¹  Miaomiao Han¹  Huancai Liu¹  Dongli Sun¹  Xiaolong Xiao² 

[1] Department of Urology, Tangshan Central Hospital, East of Guangming Road, South of Longfu South Road, West of Youyi Road West Auxiliary Road, North of Changning Road, 063000, Tangshan, China;Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai, China;

关键词: SPN;    miR-129-5p;    Clear cell renal cell carcinoma;    Proliferation;    Migration and invasion;    Apoptosis;   

DOI  :  10.1186/s12935-021-01820-3

来源: Springer

【 摘 要 】

ObjectiveOur study aims to investigate the mechanism of the miR-129-5p/SPN axis in clear cell renal cell carcinoma (ccRCC), providing a novel direction for the targeted therapy of ccRCC.MethodsBioinformatics methods were implemented to find the differentially expressed genes (DEGs) associated with ccRCC from TCGA database. qRT-PCR was performed to detect miR-129-5p and SPN mRNA expression, while western bot was carried out for the detection of protein expression of SPN. Bioinformatics analysis was used to predict the binding sites of miR-129-5p on SPN 3’UTR, while dual-luciferase assay was conducted to verify their binding relationship. CCK-8 assay, colony formation assay, wound healing assay and Transwell assay were employed to measure ccRCC cell proliferative ability, cell formation ability, cell migratory and invasive abilities. Flow cytometry was implemented to assess cell cycle and apoptosis.ResultsmiR-129-5p exhibited a significantly down-regulated expression level in ccRCC, while SPN showed a remarkably up-regulated expression level. Overexpressed miR-129-5p inhibited ccRCC cell proliferative, invasive and migratory capacities while induced cell cycle arrest in G0/G1 phase and promoted cell apoptosis. Dual-luciferase assay confirmed that there was a binding relationship between miR-129-5p and SPN. Moreover, overexpressed miR-129-5p remarkably reduced SPN expression in cancer cells, weakened the promoting effect of SPN on cell proliferation, migration, invasion and cell cycle progress, and led to enhanced cell apoptotic activity.ConclusionsOur study proves the regulatory effect of the miR-129-5p/SPN axis in ccRCC, and provides a novel potential target for precise treatment of patients with ccRCC.

【 授权许可】

CC BY