Potential role of m6A RNA methylation regulators in osteosarcoma and its clinical prognostic value

Journal of Orthopaedic Surgery and Research

Hailin Bao¹  Guangzhen Qin²  Xiaojian Wang²  Hua Liu²  Aichun Wei²  Zhigang Cai²  Xiaojun Guan²  Yanan Ji³ 

[1] Clinical Laboratory, Haian Hospital of Traditional Chinese Medicine, Haian, 226600, Nantong, Jiangsu Province, People’s Republic of China;Department of Orthopedics, Haian Hospital of Traditional Chinese Medicine, 55 Ninghai Middle Road, Haian, 226600, Nantong, Jiangsu Province, People’s Republic of China;Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, 226001, Nantong, Jiangsu Province, People’s Republic of China;

关键词: Osteosarcoma;    m6A;    m6A regulators;    Prognosis;    TCGA;   

DOI  :  10.1186/s13018-021-02422-5

来源: Springer

【 摘 要 】

BackgroundOsteosarcoma is a disease with high mortality in children and adolescents, and metastasis is one of the important clinical features of osteosarcoma. N6-Methyladenosine (m6A) is the most abundant methylation modification in mRNA, which is regulated by m6A regulators. It is reported that it is related to the occurrence and development of tumors. However, the mechanism of its action in osteosarcoma is rarely known. The purpose of this study was to identify the potential role of m6A regulatory factor in osteosarcoma and its clinical prognostic value.MethodsHere, we used The Cancer Genome Atlas (TCGA) to comprehensively analyze the relationship between m6A regulatory factors and osteosarcoma (metastasis group and non-metastasis group). We analyzed their survival relationship and analyzed all the m6A regulatory factors in TCGA tumor data set by using the univariate Cox proportional hazard regression model. Finally, we selected two survival-related methylation regulators (FTO and IGF2BP2) as risk gene signature.ResultsAccording to the median risk, patients were divided into low-risk group and high-risk group. Multivariate Cox regression analysis showed that these two risk genes were considered to be the key factors independently predicting the prognosis of patients with osteosarcoma. In addition, we verified their characteristics with gene expression omnibus (GEO) DataSets and confirmed that they are related to tumor and immune-related signaling pathways through gene set enrichment analysis (GESA) and immune infiltration analysis.ConclusionsIn conclusion, m6A regulators might play an important role in the metastasis of osteosarcoma and have potential important value for the prognosis and treatment strategy of osteosarcoma patients.

【 授权许可】

CC BY