Infant rhesus macaques as a non-human primate model of Bordetella pertussis infection

BMC Infectious Diseases

Xiao Ma¹  Chen Wei¹  Peng Luo¹  Lichan Wang¹  Shuyuan Liu²  Li Shi²  Qin Gu³  Weilun Zuo³  Na Gao³  Yan Ma³  Jiangli Liang³  Wenwen Jiang³  Dachao Mou³  Mingbo Sun³  Jingyan Li³ 

[1] Department of Diphtheria, Tetanus and Pertussis Vaccine and Toxins, National Institute for Food and Drug Control, Beijing, China;Institute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, 650118, Kunming, Yunnan, China;Institute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, 650118, Kunming, Yunnan, China;Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, 650118, Kunming, Yunnan, China;

关键词: Bordetella pertussis;    Rhesus macaques;    Infection;    Transmission;   

DOI  :  10.1186/s12879-021-06090-y

来源: Springer

【 摘 要 】

BackgroundThe prevalent resurgence of pertussis has recently become a critical public health problem worldwide. To understand pertussis pathogenesis and the host response to both the pathogen and vaccines, a suitable pertussis animal model, particularly a non-human primate model, is necessary. Recently, a non-human primate pertussis model was successfully established with baboons. Rhesus macaques have been shown to be ideal animal models for several infectious diseases, but a model of infectious pertussis has not been established in these organisms. Studies on rhesus macaque models of pertussis were performed in the 1920s–1930s, but limited experimental details are available. Recent monkey pertussis models have not been successful because the typical clinical symptoms and transmission have not been achieved.MethodsIn the present study, infant rhesus macaques were challenged with Bordetella pertussis (B.p) using an aerosol method to evaluate the feasibility of this system as an animal model of pertussis.ResultsUpon aerosol infection, monkeys infected with the recently clinically isolated B.p strain 2016-CY-41 developed the typical whooping cough, leukocytosis, bacteria-positive nasopharyngeal wash (NPW), and interanimal transmission of pertussis. Both systemic and mucosal humoral responses were induced by B.p.ConclusionThese results demonstrate that a model of pertussis was successfully established in infant rhesus macaques. This model provides a valuable platform for research on pertussis pathogenesis and evaluation of vaccine candidates.

【 授权许可】

CC BY