Adipose-derived mesenchymal stem cells from obese mice prevent body weight gain and hyperglycemia

Stem Cell Research & Therapy

Shengxian Li¹  Yicheng Qi¹  Wen Liu¹  Nan Lu¹  Wei Liu¹  Minglan Yang¹  Jing Ma¹  Xiangsheng Wang²  Wenjie Zhang² 

[1] Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiaotong University, No. 160 Pujian Road, Pudong New Area, 200127, Shanghai, China;Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai Key Laboratory of Tissue Engineering, No. 639 Zhizaoju Road, Huangpu Area, 200011, Shanghai, China;

关键词: Adipose-derived MSCs;    Lipolysis;    INSR;    Obesity;    Hyperglycemia;   

DOI  :  10.1186/s13287-021-02357-y

来源: Springer

【 摘 要 】

Changes that occur to the stem cell microenvironment with disease are a major consideration that may affect the behavior and potential therapeutic efficacy of mesenchymal stem cells (MSCs). The purpose of this study is to evaluate the effects of adipose-derived MSCs (ADSCs) from obese mice with hyperglycemia on body weight and glucose homeostasis. After 10 weeks of high-fat diet, mice were injected with phosphate-buffered saline (PBS) and ADSCs derived from normal mice (N-ADSCs) or obese mice (O-ADSCs), respectively. Mice fed with standard rodent chow were injected with PBS and served as normal controls. Obese mice treated with O-ADSCs showed less body weight gain than those receiving PBS or N-ADSCs. The mice that received ADSCs, especially O-ADSCs, also showed improvement in obesity-related hyperglycemia. In particular, the inguinal fat was reduced in obese mice receiving O-ADSCs compared with other groups, probably caused by the increased lipolysis of inguinal fat. Moreover, ADSC infusion restored insulin receptor (INSR) expression in the muscle of obese mice. Differential expression of the CD90 surface marker was slightly increased, while monocyte chemoattractant protein 1 (MCP-1) was reduced in O-ADSCs compared to N-ADSCs. These data provide a theoretical basis that autologous ADSCs from obese individuals may be more effective for treating obesity and related hyperglycemia.

【 授权许可】

CC BY