Salivary testing of COVID-19: evaluation of serological testing following positive salivary results

BMC Infectious Diseases

Lisa Caulley¹  Nadia Hua¹  Stephanie Johnson-Obaseki¹  Julie Shaw²  Jonathan Whelan³  Jonathan B. Angel⁴  Martin Corsten⁵  Kym Antonation⁶  Guillaume Poliquin⁷ 

[1] Department of Otolaryngology – Head and Neck Surgery, University of Ottawa, 501 Smyth Rd, K1H8L1, Ottawa, Canada;Department of Pathology and Laboratory Medicine, University of Ottawa, 451 Smyth Road, K1H8L1, Ottawa, Canada;Department of Undergraduate Medical Education, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H8L1, Ottawa, Canada;Division of Infectious Diseases, University of Ottawa, 451 Smyth Road, K1H8L1, Ottawa, Canada;Chronic Disease Program, Ottawa Hospital Research Institute, 1053 Carling Ave, K1H8L1, Ottawa, Canada;Division of Otolaryngology – Head and Neck Surgery, Dalhousie University, 6299 South St, B3F4R2, Halifax, Canada;National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington St, R3E3M4, Winnipeg, Canada;National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington St, R3E3M4, Winnipeg, Canada;Department of Pediatrics & Child Health, University of Manitoba, 66 Chancellors Cir, R3T2N2, Winnipeg, Canada;

关键词: Coronavirus;    COVID-19;    SARS-CoV-2;    Antibodies;    Saliva;   

DOI  :  10.1186/s12879-021-06108-5

来源: Springer

【 摘 要 】

BackgroundSalivary detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proposed as an alternative to nasopharyngeal or oropharyngeal swab testing. Our group previously published a study demonstrating that both testing methods identified SARS-CoV-2 using polymerase chain reaction (PCR)-based detection methodology. We therefore conducted a follow-up study using antibody testing to evaluate the accuracy of saliva versus swabs for COVID-19 detection and the durability of antibody response.MethodsVenous blood samples were collected from consenting participants and the presence of serum antibodies for SARS-CoV-2 was evaluated on a large, automated immunoassay platform by the Roche anti-SARS-CoV-2 qualitative assay (Roche Diagnostics, Laval Quebec). Individuals with a serum antibody cut-off index (COI) ≥ 1.0 were considered positive.ResultsIn asymptomatic and mildly symptomatic patients with a previously positive standard swab and/or saliva SARS-CoV-2 PCR-test, 42 demonstrated antibodies with 13 patients positive by swab alone, and 8 patients positive by saliva alone.ConclusionsDespite their status as ‘current standard’ for COVID-19 testing, these findings highlight limitations of PCR-based tests.

【 授权许可】

CC BY