An ionising radiation-induced specific transcriptional signature of inflammation-associated genes in whole blood from radiotherapy patients: a pilot study

Radiation Oncology

Lone Gothard¹  Selvakumar Anbalagan¹  Navita Somaiah¹  Lourdes Cruz-Garcia²  Jayne Moquet²  Grainne O’Brien²  Christophe Badie³  Elizabeth A. Ainsbury³ 

[1] Institute of Cancer Research and The Royal Marsden NHS Foundation Trust, SM2 5NG, Sutton, London, UK;PHE CRCE, Chilton, Didcot, OX11 0RQ, Oxford, UK;PHE CRCE, Chilton, Didcot, OX11 0RQ, Oxford, UK;Environmental Research Group within the School of Public Health, Faculty of Medicine at Imperial College of Science, Technology and Medicine, London, UK;

关键词: Ionising radiation;    Blood;    Gene expression;    Transcription;    NCounter;    RT-qPCR;    Inflammation;    Radiotherapy;    Cancer;   

DOI  :  10.1186/s13014-021-01807-4

来源: Springer

【 摘 要 】

BackgroundThis communication reports the identification of a new panel of transcriptional changes in inflammation-associated genes observed in response to ionising radiation received by radiotherapy patients.MethodsPeripheral blood samples were taken with ethical approval and informed consent from a total of 20 patients undergoing external beam radiotherapy for breast, lung, gastrointestinal or genitourinary tumours. Nanostring nCounter analysis of transcriptional changes was carried out in samples prior and 24 h post-delivery of the 1st radiotherapy fraction, just prior to the 5th or 6th fraction, and just before the last fraction.ResultsStatistical analysis with BRB-ArrayTools, GLM MANOVA and nSolver, revealed a radiation responsive panel of genes which varied by patient group (type of cancer) and with time since exposure (as an analogue for dose received), which may be useful as a biomarker of radiation response.ConclusionFurther validation in a wider group of patients is ongoing, together with work towards a full understanding of patient specific responses in support of personalised approaches to radiation medicine.

【 授权许可】

CC BY