Impact of mixed meal tolerance test composition on measures of beta-cell function in type 2 diabetes

Nutrition & Metabolism

Oana-Patricia Zaharia¹  Michael Roden¹  Kálmán Bódis¹  Theresa Kössler¹  Julia Szendroedi²  Klaus Strassburger³  Pavel Bobrov³  Oliver Kuss³  Yanislava Karusheva⁴  Clara Möser⁴  Volker Burkart⁴ 

[1] Division of Endocrinology and Diabetology, Medical Faculty, Heinrich Heine University, Moorenstr. 5, 40225, Düsseldorf, Germany;Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research At Heinrich Heine University Düsseldorf, Düsseldorf, Germany;German Center for Diabetes Research (DZD), München-Neuherberg, Germany;Division of Endocrinology and Diabetology, Medical Faculty, Heinrich Heine University, Moorenstr. 5, 40225, Düsseldorf, Germany;Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research At Heinrich Heine University Düsseldorf, Düsseldorf, Germany;German Center for Diabetes Research (DZD), München-Neuherberg, Germany;Department of Internal Medicine I and Clinical Chemistry, University Hospital Heidelberg, Heidelberg, Germany;German Center for Diabetes Research (DZD), München-Neuherberg, Germany;Institute for Biometrics and Epidemiology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf, Düsseldorf, Germany;Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research At Heinrich Heine University Düsseldorf, Düsseldorf, Germany;German Center for Diabetes Research (DZD), München-Neuherberg, Germany;

关键词: Mixed meal tolerance test;    Type 2 diabetes;    Intra-individual variation;    Inter-individual variation;    Beta-cell function;   

DOI  :  10.1186/s12986-021-00556-1

来源: Springer

【 摘 要 】

BackgroundApplication of mixed meal tolerance tests (MMTT) to measure beta-cell function in long-term studies is limited by modification of the commercial products occurring over time. This study assessed the intra-individual reliability of MMTTs and compared the effects of liquid meals differing in macronutrient composition on the estimation of beta-cell function in type 2 diabetes (T2DM).MethodsTo test the reliability of MMTTs, 10 people with T2DM (age 58 ± 11 years, body mass index 30.0 ± 4.9 kg/m2) received Boost®high Protein 20 g protein three times. For comparing different meals, another 10 persons with T2DM (58 ± 5 years, 31.9 ± 5.3 kg/m2) ingested either Boost®high Protein 20 g protein or the isocaloric Boost®high Protein 15 g protein containing 35% less protein and 18% more carbohydrates. C-peptide, insulin and glucose release were assessed from the incremental area under the concentration time curve (iAUC) and the intra- and inter-individual variation of these parameters from the coefficients of variations (CV).ResultsRepetitive ingestion of one meal revealed intra-individual CVs for the iAUCs of C-peptide, insulin and glucose, which were at least 3-times lower than the inter-individual variation of these parameters (18.2%, 19.7% and 18.9% vs. 74.2%, 70.5% and 207.7%) indicating a good reliability. Ingestion of two different meals resulted in comparable intra-individual CVs of the iAUCs of C-peptide and insulin (16.9%, 20.5%).ConclusionMMTTs provide reliable estimation of beta-cell function in people with T2DM. Furthermore, moderate differences in the protein and carbohydrate contents in a standardized liquid meal do not result in relevant changes of C-peptide and insulin responses.Trial registration: Clinicaltrials.gov, Identifier number: NCT01055093. Registered 22 January 2010 – Retrospectively registered, https://www.clinicaltrials.gov/ct2/show/study/NCT01055093

【 授权许可】

CC BY