Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria

BMC Microbiology

Noor S. K. Al-Khafaji¹  Hussein O. M. Al-Dahmoshi¹  Emmanuel Nwakaeze²  Ifeanyichukwu Iroha²  Chika Ejikeugwu²  Okoro Nworie³  Peter Eze⁴  Chika Ezeador⁵  Morteza Saki⁶  Eniola Oni⁷  Chidiebere Obi⁸  Charles Esimone⁹  Michael U. Adikwu^1,10 

[1] Biology Department, College of Science, University of Babylon, Hilla City, Babylon Province, Iraq;Department of Applied Microbiology, Ebonyi State University, Abakaliki, Nigeria;Department of Biological Sciences, Alex Ekwueme Federal University, Ndufu-Alike Ikwo, Ikwo, Nigeria;Department of Environmental Health Science, Nnamdi Azikiwe University, Awka, Nigeria;Department of Medical Microbiology & Parasitology, Nnamdi Azikiwe University, Awka, Nigeria;Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;Department of Microbiology, Federal University of Agriculture, Abeokuta, Nigeria;Department of Microbiology, Federal University, Birnin Kebbi, Nigeria;Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Awka, Nigeria;Department of Pharmaceutics, University of Nigeria, Nsukka, Nigeria;

关键词: AmpC;    Antibiotic resistance;    Gram-negative bacteria;    Metallo-β-lactamase;    Multidrug resistance;    Nigeria;   

DOI  :  10.1186/s12866-021-02179-1

来源: Springer

【 摘 要 】

BackgroundGram-negative bacteria (GNB) including Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae represent the most relevant reservoir of resistance genes such as metallo-β-lactamase (MBL) and AmpC genes that give them the undue advantage to resist antimicrobial onslaught. This study aimed to investigate the occurrence of MBL (blaIMP-1, blaIMP-2, blaVIM-1, blaVIM-2) and AmpC (blaFOX, blaDHA, blaCMY, blaACC) resistance genes in aforementioned GNB collected from abattoir and poultry sources in Nigeria.ResultsIn total, 370 isolates were collected from abattoir tables (n = 130), anal region of cows (n = 120), and the cloacae of poultry birds (n = 120). The test isolates showed high rate of resistance to cephalosporins and carbapenems. The MBLs were phenotypically detected in 22 E. coli, 22 P. aeruginosa, and 18 K. pneumoniae isolates using combined disc test (CDT). However, only 11 E. coli, 24 P. aeruginosa, and 18 Klebsiella pneumoniae isolates were phenotypically confirmed to be AmpC producers using cefoxitin-cloxacillin double disk synergy test (CC-DDST). MBL encoding genes (particularly the blaIMP-1 genes and blaIMP-2 genes) were detected by polymerase chain reaction (PCR) in 12 (54.6%) E. coli, 15 (83.3%) K. pneumoniae, and 16 (72.7%) P. aeruginosa isolates. AmpC genes (particularly the blaCMY genes and blaFOX genes) were found in a total of 5 (29.4%) E. coli isolates, 5 (27.8%) isolates of K. pneumoniae, and 10 (41.7%) isolates of P. aeruginosa.ConclusionsOur study showed the circulation of MBL and AmpC genes in GNB from abattoir and poultry origin in Nigeria. Adoption of regular control policies is necessary to reduce the spread of these species as soon as possible, especially in poultry and slaughterhouses.

【 授权许可】

CC BY