期刊论文详细信息
Journal of Translational Medicine
Autism-associated synaptic vesicle transcripts are differentially expressed in maternal plasma exosomes of physiopathologic pregnancies
Mei Zhong1  Yangwu Fang2  Youlu Wen3  Chan Wan4  Jing Pan4  Ze Wu4  Nanbert Zhong5 
[1] Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, China;Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, China;Sino-US Center of Translational Medicine for Development Disabilities, Southern Medical University, 510515, Guangzhou, China;Department of Psychiatry, Guangdong 999 Brain Hospital, 510510, Guangzhou, China;Sino-US Center of Translational Medicine for Development Disabilities, Southern Medical University, 510515, Guangzhou, China;Sino-US Center of Translational Medicine for Development Disabilities, Southern Medical University, 510515, Guangzhou, China;Department of Human Genetics, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Rd, 10314, Staten Island, NY, USA;
关键词: Synapse;    Spontaneous preterm birth;    Preeclampsia;    Gestational diabetes mellitus;    Exosomes;   
DOI  :  10.1186/s12967-021-02821-6
来源: Springer
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【 摘 要 】

BackgroundDuring intrauterine development, the formation and function of synaptic vesicles (SVs) are thought to be fundamental conditions essential for normal development of the brain. Lacking advanced technology during the intrauterine period, such as longitudinal real-time monitoring of the SV-associated transcripts (SVATs), which include six pairs of lncRNA-mRNA, has limited acquisition of the dynamic gene expression profile (GEP) of SVATs. We previously reported the differential expression of SVATs in the peripheral blood of autistic children. The current study was designed to determine the dynamic profiles of differentially-expressed SVATs in circulating exosomes (EXs) derived from autistic children and pregnant women at different gestational ages.MethodsBlood samples were collected from autistic children and women with variant physiopathologic pregnancies. EXs were isolated with an ExoQuick Exosome Precipitation Kit and characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western blotting. The expression of lncRNAs and lncRNA-targeted mRNAs were quantified using real-time PCR.ResultsSVAT-associated lncRNAs-mRNAs were detected in autistic children and differentially expressed from the first trimester of pregnancy to the term of delivery. Pathologic pregnancies, including spontaneous preterm birth (sPTB), preeclampsia (PE), and gestational diabetes mellitus (GDM), were compared to normal physiologic pregnancies, and shown to exhibit specific correlations between SVAT-lncRNA and SVAT-mRNA of STX8, SLC18A2, and SYP with sPTB; SVAT-lncRNA and SVAT-mRNA of STX8 with PE; and SVAT-lncRNA and SVAT-mRNA of SV2C as well as SVAT-mRNA of SYP with GDM.ConclusionVariant complications in pathologic pregnancies may alter the GEP of SVATs, which is likely to affect the intrauterine development of neural circuits and consequently influence fetal brain development.

【 授权许可】

CC BY   

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