Carbohydrate-binding modules targeting branched polysaccharides: overcoming side-chain recalcitrance in a non-catalytic approach

Bioresources and Bioprocessing

Jingrong Zhu¹  Jiawen Liu¹  Di Sun¹  Weijie Liu¹  Cong Liu¹ 

[1] Jiangsu Key Laboratory of Phylogenomics & Comparative Genomics, School of Life Science, Jiangsu Normal University, No. 101, Shanghai Road, Tongshan New District, 221116, Xuzhou, Jiangsu, China;

关键词: Carbohydrate-binding module;    Protein structure;    Protein-carbohydrate recognition;    CBM fusion;    Hemicellulose;    Side-chain recalcitrance;    Lignocellulose conversion;   

DOI  :  10.1186/s40643-021-00381-7

来源: Springer

【 摘 要 】

Extensive decoration of backbones is a major factor resulting in resistance of enzymatic conversion in hemicellulose and other branched polysaccharides. Employing debranching enzymes is the main strategy to overcome this kind of recalcitrance at present. A carbohydrate-binding module (CBM) is a contiguous amino acid sequence that can promote the binding of enzymes to various carbohydrates, thereby facilitating enzymatic hydrolysis. According to previous studies, CBMs can be classified into four types based on their preference in ligand type, where Type III and IV CBMs prefer to branched polysaccharides than the linear and thus are able to specifically enhance the hydrolysis of substrates containing side chains. With a role in dominating the hydrolysis of branched substrates, Type III and IV CBMs could represent a non-catalytic approach in overcoming side-chain recalcitrance.

【 授权许可】

CC BY