Lymphocyte expansion in bioreactors: upgrading adoptive cell therapy

Journal of Biological Engineering

Christoph Herwig¹  Oscar Fabian Garcia-Aponte¹  Bence Kozma¹ 

[1] Research Area Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorferstraße 1a, 1060, Vienna, Austria;

关键词: Adoptive cell therapy;    ATMP;    Bioreactor;    Expansion;    Lymphocyte;    Rocking motion;    Stirred reactor;    Perfusion reactor;    NK cell;    T cell;   

DOI  :  10.1186/s13036-021-00264-7

来源: Springer

【 摘 要 】

Bioreactors are essential tools for the development of efficient and high-quality cell therapy products. However, their application is far from full potential, holding several challenges when reconciling the complex biology of the cells to be expanded with the need for a manufacturing process that is able to control cell growth and functionality towards therapy affordability and opportunity. In this review, we discuss and compare current bioreactor technologies by performing a systematic analysis of the published data on automated lymphocyte expansion for adoptive cell therapy. We propose a set of requirements for bioreactor design and identify trends on the applicability of these technologies, highlighting the specific challenges and major advancements for each one of the current approaches of expansion along with the opportunities that lie in process intensification. We conclude on the necessity to develop targeted solutions specially tailored for the specific stimulation, supplementation and micro-environmental needs of lymphocytes’ cultures, and the benefit of applying knowledge-based tools for process control and predictability.

【 授权许可】

CC BY