Intraperitoneal injection of lithium chloride induces lateralized activation of the insular cortex in adult mice

Molecular Brain

Shuang Qiu¹  Kai Qian²  Yiqing Cao²  Jiaqi Liu³  Jing Yang³ 

[1] Department of Neurobiology, Department of Anesthesiology of Second Affiliated Hospital, Zhejiang University School of Medicine, 310058, Hangzhou, China;NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, 310058, Hangzhou, China;NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, 310058, Hangzhou, China;School of Life Sciences, Center for Life Sciences, IDG/McGovern Institute for Brain Research, Peking University, 100871, Beijing, China;

关键词: Insular cortex;    Lateralization;    Vagotomy;    LiCl;    Fos staining;   

DOI  :  10.1186/s13041-021-00780-z

来源: Springer

【 摘 要 】

Insular cortex is a critical brain region that participates in the interoceptive sensations. Here, we combined the iDISCO + method and Fos immunostaining to confirm that the middle part of the right-side, but not the left-side, insular cortex in adult male mice is activated by intraperitoneal injection of lithium chloride. Lateralized activation of the insular cortex is also observed in adult female mice, but not in young or aged male mice. Furthermore, asymmetrical activation of the insular cortex was completely blocked when both sides of the vagal nerve are transected, whereas intravenous injection of lithium chloride has no effect on the insular activation. Combined together, these results indicate that the insular cortex unilaterally responds to aversive visceral stimuli in an age-dependent way and this process depends on the vagal afferent pathways.

【 授权许可】

CC BY