Viral load and high prevalence of HR-HPV52 and 58 types in black women from rural communities

BMC Infectious Diseases

Jerusa Araújo Dias¹  Maria Carmen Lopes Ferreira Silva²  Lays Paula Bondi Volpini³  Liliana Cruz Spano⁴  Angélica Espinosa Miranda⁵  Luciana Bueno de Freitas⁶ 

[1] Department of Nursing, University Center of Northern Espírito Santo, Federal University of Espírito Santo, São Mateus, Brazil;Department of Pathology, Center of Health Sciences, Federal University of Espírito Santo, Vitória, Brazil;Infectious Diseases Post-Graduate Program, Federal University of Espírito Santo, Vitória, Brazil;Infectious Diseases Post-Graduate Program, Federal University of Espírito Santo, Vitória, Brazil;Department of Pathology, Center of Health Sciences, Federal University of Espírito Santo, Vitória, Brazil;Infectious Diseases Post-Graduate Program, Federal University of Espírito Santo, Vitória, Brazil;Department of Social Medicine, Center of Health Sciences, Federal University of Espírito Santo, Vitória, Brazil;Multivix Faculty, Vitória, Brazil;

关键词: HPV;    Viral load;    Rural communities;    Cervical cancer screening;   

DOI  :  10.1186/s12879-021-06042-6

来源: Springer

【 摘 要 】

BackgroundThe high-risk human papillomavirus (HR-HPV) infection is the main cause of cervical cancer development, and the most common types were included in the last approved nonavalent vaccine (9vHPV). Geographical, socioeconomic and ethnic barriers in developing countries challenge primary and secondary prevention measures of cervical cancer. We aimed to determine the prevalence of HPV infection and the viral load of HR-HPV 9vHPV-related types black women resident in rural semi-isolated communities.MethodsA descriptive study was conducted with 273 cervical samples of women from rural communities of Southeastern Brazil. Viral DNA was amplified by PCR, the genotype was identified by Reverse Line Blot (RLB) and Restriction Fragment Length Polymorphism (RFLP), and real-time PCR was applied to determine the viral load.ResultsHPV frequency was 11.4% (31/273), associated with the presence of cytological abnormalities (32.3%; p < 0.001). Thirty-one distinct genotypes were detected; HR-HPV occurred in 64.5% (20/31) of the samples and the most prevalent type were HPV52 > 58, 59. Multiple infections occurred with up to nine different genotypes. The viral load of HR-HPV 9vHPV-related types was higher in lesions than in normal cytology cases (p = 0.04); “high” and “very high” viral load occurred in HSIL and LSIL, respectively (p = 0.04).ConclusionsWe highlight that despite the low HPV frequency in the black rural women population, the frequency of HR-HPV was high, particularly by the HR-HPV52 and 58 types. Moreover, the HR-HPV viral load increased according to the progression from normal to lesion, being a potential biomarker to identify those women at higher risk of developing cervical lesions in this population.

【 授权许可】

CC BY