Determining vector competence of Aedes aegypti from Ghana in transmitting dengue virus serotypes 1 and 2

Parasites & Vectors

Faustus Akankperiwen Azerigyik¹  Michael Amoa-Bosompem²  Shiroh Iwanaga³  Mitsuko Ohashi⁴  Kyoko Sawabe⁵  Haruhiko Isawa⁵  Daisuke Kobayashi⁵  Yoshihide Maekawa⁵  Shinji Kasai⁵  Astri Nur Faizah⁶  Katsunori Murota⁷  Ryosuke Fujita⁸  Shoji Yamaoka⁹  Samuel Dadzie^1,10  Joseph H. Kofi Bonney^1,11  Takaya Hayashi^1,12  Nobuo Ohta^1,13  Phong Vu Tran^1,14  Cuong Chi Tran^1,14  Kentaro Itokawa^1,15 

[1]Department of Environmental Parasitology, Tokyo Medical and Dental University, Bunkyo, Tokyo, Japan

[2]Department of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan

[3]Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, College of Health Sciences, Legon, Accra, Ghana

[4]Department of Environmental Parasitology, Tokyo Medical and Dental University, Bunkyo, Tokyo, Japan

[5]Department of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan

[6]Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, College of Health Sciences, Legon, Accra, Ghana

[7]Laboratory of Sanitary Entomology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan

[8]Department of Environmental Parasitology, Tokyo Medical and Dental University, Bunkyo, Tokyo, Japan

[9]Department of Molecular Protozoology, Research Center for Infectious Disease Control, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan

[10]Department of Environmental Parasitology, Tokyo Medical and Dental University, Bunkyo, Tokyo, Japan

[11]Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, College of Health Sciences, Legon, Accra, Ghana

[12]Department of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan

[13]Department of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan

[14]Graduate School of Agricultural and Life Science, The University of Tokyo, Bunkyo, Tokyo, Japan

[15]Department of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan

[16]Kyushu Research Station, National Institute of Animal Health, NARO, Chuzan, Kagoshima, Japan

[17]Department of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan

[18]Laboratory of Sanitary Entomology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan

[19]Department of Molecular Virology, Tokyo Medical and Dental University, Bunkyo, Tokyo, Japan

[20]Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, College of Health Sciences, Legon, Accra, Ghana

[21]Department of Virology, Noguchi Memorial Institute for Medical Research, University of Ghana, College of Health Sciences, Legon, Accra, Ghana

[22]Department of Virology, Noguchi Memorial Institute for Medical Research, University of Ghana, College of Health Sciences, Legon, Accra, Ghana

[23]Department of Molecular Virology, Tokyo Medical and Dental University, Bunkyo, Tokyo, Japan

[24]Faculty of Health Science, Suzuka University of Medical Science, Suzuka, Mie, Japan

[25]Medical Entomology and Zoology Department, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam

[26]Pathogen Genomics Center, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan

关键词: Aedes aegypti;    Vector competence;    Dengue virus;    Susceptibility;    Ghana;    West Africa;   

DOI  :  10.1186/s13071-021-04728-z

来源: Springer

【 摘 要 】

BackgroundDengue virus (DENV) is a mosquito-borne arbovirus transmitted by Aedes mosquitoes, but is not endemic in all areas where this vector is found. For example, the relatively sparse distribution of cases in West Africa is generally attributed to the refractory nature of West African Aedes aegypti (Ae. aegypti) to DENV infection, and particularly the forest-dwelling Ae. aegypti formosus. However, recent studies have shown these mosquitoes to be competent vectors within some West African countries that have suffered outbreaks in the past, such as Senegal. There is however little information on the vector competence of the Ae. aegypti in West African countries such as Ghana with no reported outbreaks.MethodsThis study examined the vector competence of 4 Ae. aegypti colonies from urban, semi-urban, and two rural locations in Ghana in transmitting DENV serotypes 1 and 2, using a single colony from Vietnam as control. Midgut infection and virus dissemination were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), while the presence and concentration of DENV in the saliva of infectious mosquitoes was determined by the focus forming assay.ResultsThere were significant differences in the colonies’ susceptibility to virus infection, dissemination, and transmission. All examined Ghanaian mosquitoes were refractory to infection by DENV serotype 2, while some colonies exhibited potential to transmit DENV serotype 1. None of the tested colonies were as competent as the control group colony.ConclusionsThese findings give insight into the possible risk of outbreaks, particularly in the urban areas in the south of Ghana, and highlight the need for continuous surveillance to determine the transmission status and outbreak risk. This study also highlights the need to prevent importation of different DENV strains and potential invasion of new highly vector-competent Ae. aegypti strains, particularly around the ports of entry.Graphic Abstract

【 授权许可】

CC BY