Three‐month outcomes of aortic valve reconstruction using collagenous membranes (biosheets) produced by in‐body tissue architecture in a goat model: a preliminary study

BMC Cardiovascular Disorders

Yasuhide Nakayama¹  Hirofumi Anai²  Keitaro Okamoto³  Tadashi Umeno⁴  Tomoyuki Wada⁴  Shinji Miyamoto⁴  Takashi Shuto⁴  Haruto Nishida⁵ 

[1] Biotube Co., Ltd, Tokyo, Japan;Clinical Engineering, Faculty of Medicine, Oita University, Yufu, Oita, Japan;Department of Cardiovascular Surgery, Faculty of Medicine, Oita University, 1-1 Idaigaoka, 879-5593, Yufu, Oita, Japan;Department of Cardiovascular Surgery, Oita University Hospital, Oita, Japan;Department of Cardiovascular Surgery, Oita University Hospital, Oita, Japan;Department of Pathology, Oita University Hospital, Oita, Japan;

关键词: Aortic root;    Valve repair;    Tissue Engineering;    Valve replacement;   

DOI  :  10.1186/s12872-021-01988-6

来源: Springer

【 摘 要 】

BackgroundAutologous pericardium is widely used as a plastic material in intracardiac structures, in the pulmonary artery, and in aortic valve leaflets. For aortic valve reconstruction (AVRec) using the Ozaki procedure, it has produced excellent clinical results over a 10-year period. In-body tissue architecture (iBTA), which is based on the phenomenon of tissue encapsulation of foreign materials, can be used to prepare autologous prosthetic tissues. In this preliminary study, we examined whether biosheets can be used as valve leaflet material for glutaraldehyde-free AVRec by subchronic implantation experiments in goats and evaluated its performance compared with glutaraldehyde-treated autologous pericardium for AVRec.MethodsBiosheets were prepared by embedding molds for two months into the dorsal subcutaneous spaces of goats. Autogenic biosheets (n = 4) cut into the shape of the valve were then implanted to the aortic valve annulus of four goats for three months without glutaraldehyde treatment. Autologous pericardium (n = 4) was used in four goats as a control. Valve function was observed using echocardiography.ResultsAll goats survived the three-month study period. With biosheets, the leaflet surfaces were very smooth and, on histology, partially covered with a thin neointima (including endothelial cells). Biosheets were more thoroughly assimilated into the aortic root compared with autologous pericardium.ConclusionsFor the first time, biosheets were used for large animal AVRec. Biosheets could function as leaflets in the aortic position and may have the ability to assimilate into native valves.

【 授权许可】

CC BY