l-Lactic acid production from fructose by chitosan film-coated sodium alginate-polyvinyl alcohol immobilized Lactobacillus pentosus cells and its kinetic analysis

Bioresources and Bioprocessing

Jinyue Qiao¹  Hujie Lv¹  Shaoming Jiang¹  Hebei Lin¹  Shibo Hou¹  Shijie Liu¹  Xingyu Chen¹  Yuming Lu¹  Huanyu Guo¹  Jianfei Wang¹  Ruiyi Yang¹  Xudong Bi²  Maolin Hou³  Jiaqi Huang⁴ 

[1]Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, NY13210, Syracuse, USA

[2]Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, NY13210, Syracuse, USA

[3]California State University, Los Angeles (CSULA), 90032, Los Angeles, CA, USA

[4]Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, NY13210, Syracuse, USA

[5]Department of Biomedical Engineering, City University of Hong Kong, 999077, Hong Kong, China

[6]Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, NY13210, Syracuse, USA

[7]The Center for Biotechnology and Interdisciplinary Studies (CBIS) at Rensselaer Polytechnic Institute, NY12180, Troy, USA

关键词: Cell immobilization;    Kinetics;    Optimization;    Batch fermentation;    Lactic acid;   

DOI  :  10.1186/s40643-021-00380-8

来源: Springer

【 摘 要 】

Under the optimal conditions of immobilization and fermentation, the highest LA yield of 0.966 ± 0.006 g/g fructose and production rate of 2.426 ± 0.018 g/(L × h) with an error of -0.5% and -0.2% to the predicted results were obtained from batch fermentation by the CS film-coated SA-PVA immobilized L. pentosus cells. The LA yield and production rate of these immobilized cells were 2.7% and 10.1% higher than that of normal SA-PVA immobilized cells respectively, and they were 5.7% and 48.4% higher than that of free cells, respectively. The effect of temperature on different types of immobilized cells and free cells was significantly different, but the effect of pH on different types of cells was not much different. The kinetic models could effectively describe the different fermentation performances of three types of cells. The immobilized cells have excellent reusability to conduct 9 runs of repeated batch fermentation.

【 授权许可】

CC BY