Multi-scale comparative transcriptome analysis reveals key genes and metabolic reprogramming processes associated with oil palm fruit abscission

BMC Plant Biology

Jean-Luc Verdeil¹  Chatchawan Jantasuriyarat²  Sithichoke Tangphatsornruang³  Philippe Amblard⁴  Myriam Collin⁵  Thierry Joët⁵  Stéphane Dussert⁵  Julien Serret⁵  Timothy J. Tranbarger⁵  Fabienne Morcillo⁶  Peerapat Roongsattham⁷  Kim Fooyontphanich⁸ 

[1] CIRAD, UMR AGAP, F-34398, Montpellier, France;AGAP, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, France;Department of Genetics, Faculty of Science, Kasetsart University Bangkhen Campus, 50 Phahonyothin Road Jatujak, Bangkok, Thailand;National Science and Technology Development Agency, 111 Thailand Science Park, Phahonyothin Road, Pathum Thani, Thailand;PalmElit SAS, Montferrier-sur-Lez, France;UMR DIADE, Institut de Recherche Pour le Développement, Université de Montpellier, IRD Centre de Montpellier, 911 Avenue Agropolis BP 64501, 34394 Cedex 5, Montpellier, France;UMR DIADE, Institut de Recherche Pour le Développement, Université de Montpellier, IRD Centre de Montpellier, 911 Avenue Agropolis BP 64501, 34394 Cedex 5, Montpellier, France;CIRAD, DIADE, F-34398, Montpellier, France;UMR DIADE, Institut de Recherche Pour le Développement, Université de Montpellier, IRD Centre de Montpellier, 911 Avenue Agropolis BP 64501, 34394 Cedex 5, Montpellier, France;Department of Genetics, Faculty of Science, Kasetsart University Bangkhen Campus, 50 Phahonyothin Road Jatujak, Bangkok, Thailand;UMR DIADE, Institut de Recherche Pour le Développement, Université de Montpellier, IRD Centre de Montpellier, 911 Avenue Agropolis BP 64501, 34394 Cedex 5, Montpellier, France;Grow A Green Co, Ltd. 556 Maha Chakraphat Rd. Namaung, 24000, Chachoengsao, Chachoengsao Province, Thailand;

关键词: Transcriptome;    Fruit abscission;    Abscission zone;    Monocotyledon;    Metabolic reprogramming;    Elaeis guineensis;   

DOI  :  10.1186/s12870-021-02874-1

来源: Springer

【 摘 要 】

BackgroundFruit abscission depends on cell separation that occurs within specialized cell layers that constitute an abscission zone (AZ). To determine the mechanisms of fleshy fruit abscission of the monocot oil palm (Elaeis guineensis Jacq.) compared with other abscission systems, we performed multi-scale comparative transcriptome analyses on fruit targeting the developing primary AZ and adjacent tissues.ResultsCombining between-tissue developmental comparisons with exogenous ethylene treatments, and naturally occurring abscission in the field, RNAseq analysis revealed a robust core set of 168 genes with differentially regulated expression, spatially associated with the ripe fruit AZ, and temporally restricted to the abscission timing. The expression of a set of candidate genes was validated by qRT-PCR in the fruit AZ of a natural oil palm variant with blocked fruit abscission, which provides evidence for their functions during abscission. Our results substantiate the conservation of gene function between dicot dry fruit dehiscence and monocot fleshy fruit abscission. The study also revealed major metabolic transitions occur in the AZ during abscission, including key senescence marker genes and transcriptional regulators, in addition to genes involved in nutrient recycling and reallocation, alternative routes for energy supply and adaptation to oxidative stress.ConclusionsThe study provides the first reference transcriptome of a monocot fleshy fruit abscission zone and provides insight into the mechanisms underlying abscission by identifying key genes with functional roles and processes, including metabolic transitions, cell wall modifications, signalling, stress adaptations and transcriptional regulation, that occur during ripe fruit abscission of the monocot oil palm. The transcriptome data comprises an original reference and resource useful towards understanding the evolutionary basis of this fundamental plant process.

【 授权许可】

CC BY