Quaternary ammonium silane (k21) based intracanal medicament triggers biofilm destruction

BMC Oral Health

Shu Fen Ong¹  Xian Jin Lim¹  Siao Hua Lim¹  Ling Ang Wong¹  Soo Xiong Chew¹  Esther Sook Kuan Kok¹  Umer Daood¹  Lok Yin See¹  Venkateshbabu Nagendrababu²  Fabian Davamani³  Amr Fawzy⁴ 

[1] Division of Clinical Dentistry, Schoolof Dentistry, International Medical University Kuala Lumpur, Kuala Lumpur, Malaysia;Division of Clinical Dentistry, Schoolof Dentistry, International Medical University Kuala Lumpur, Kuala Lumpur, Malaysia;Department of Preventive and Restorative Dentistry, College of Dental Medicine, University of Sharjah, Sharjah, UAE;Faculty of Biomedical Science, School of Health Sciences, International Medical University, Kuala Lumpur, Malaysia;UWA Dental School, University of Western Australia, Nedlands, Australia;

关键词: Calcium hydroxide;    Chlorhexidine;    Dentine;    Intracanal medicament;    Quaternary ammonium silane;   

DOI  :  10.1186/s12903-021-01470-x

来源: Springer

【 摘 要 】

BackgroundCompare antimicrobial efficacy of a quarternary ammonium silane (QAS)/k21 as an intracanal medicament against E. faecalis and C. albicans biofilms formed on root dentin.MethodologyDentin blocks were sterilized and E. faecalis and C. albicans microbial colonies were counted for colony-forming-units against 2%k21, 2%CHX and Ca(OH)2 medicaments. Biofilm colonies after 7 days on dentin were analysed using confocal laser scanning microscopy with live/dead bacterial viability staining. TEM was done to study dentin collagen matrix. Dentin discs from 3rd day and 7th day well plate was used for Raman spectra and observed under fluorescent-microscope. Docking studies were carried out on MMP-2 S1 binding-domain with k21.ResultsThere was reduction of E. faecalis/C. albicans when k21, chlorhexidine and calcium hydroxide were used with highest percentage in 2%k21 treated specimens. 2%k21 showed dense and regular collagen network with intact cross-banding and decreased Raman intensity for 2%k21 on 3rd day. NaOCl + k21 showed least adherence, whereas saline groups showed highest adherence of E. faecalis and C. albicans to root-canal dentin. Alizarin red staining of hDPSCs revealed calcium deposition in all groups with significant difference seen amongst 2%k21 groups. MMP-2 ligand binding was seen accurately indicating possible target sites for k21 intervention.Conclusion2%k21 can be considered as alternative intracanal medicament.

【 授权许可】

CC BY