Strain-specific behavior of Mycobacterium tuberculosis in A549 lung cancer cell line

BMC Bioinformatics

Parisa Riahi¹  Shayan Mostafaei²  Abolfazl Fateh³  Shima Hadifar³  Seyed Davar Siadat³  Ava Behrouzi³  Farzam Vaziri³ 

[1] Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;Department of Biostatistics, School of Health, Kermanshah University of Medical Sciences, Kermanshah, Iran;Epidemiology and Biostatistics Unit, Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran;Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran;Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran;

关键词: Mycobacterium tuberculosis;    Lung Cancer;    Lineage;    Elastic net penalized logistic regression;   

DOI  :  10.1186/s12859-021-04100-z

来源: Springer

【 摘 要 】

BackgroundA growing body of evidence has shown the association between tuberculosis (TB) infection and lung cancer. However, the possible effect of strain‐specific behavior of Mycobacterium tuberculosis (M.tb) population, the etiological agent of TB infection in this association has been neglected. In this context, this study was conducted to investigate this association with consideration of the genetic background of strains in the M.tb population.ResultsWe employed the elastic net penalized logistic regression model, as a statistical-learning algorithm for gene selection, to evaluate this association in 129 genes involved in TLRs and NF-κB signaling pathways in response to two different M.tb sub-lineage strains (L3-CAS1and L 4.5). Of the 129 genes, 21 were found to be associated with the two studied M.tb sub-lineages. In addition, MAPK8IP3 gene was identified as a novel gene, which has not been reported in previous lung cancer studies and may have the potential to be recognized as a novel biomarker in lung cancer investigation.ConclusionsThis preliminary study provides new insights into the mechanistic association between TB infection and lung cancer. Further mechanistic investigations of this association with a large number of M.tb strains, encompassing the other main M.tb lineages and using the whole transcriptome of the host cell are inevitable.

【 授权许可】

CC BY