Transcriptomic analysis of the trade-off between endurance and burst-performance in the frog Xenopus allofraseri

BMC Genomics

Thibault Scalvenzi¹  Nicolas Pollet¹  Karen A. Moore²  Konrad Paszkiewicz²  Mathieu Videlier³  Adam J. Richards⁴  Camille Bonneaud⁵  Anthony Herrel⁶  Valérie Ducret⁷ 

[1] Evolution, Génomes, Comportement & Ecologie, Université Paris-Saclay, CNRS, IRD, 91198, Gif-sur-Yvette, France;Exeter Sequencing Service, College of Life and Environmental Sciences, University of Exeter, EX4 4QD, Exeter, UK;Functional Ecology Lab, Department of Biology, University of Ottawa, 30 Marie Curie, K1N 6N5, Ottawa, ON, Canada;Station d’Ecologie Expérimentale du CNRS, USR 2936, 09200, Moulis, France;Station d’Ecologie Expérimentale du CNRS, USR 2936, 09200, Moulis, France;Centre for Ecology & Conservation, College of Life and Environmental Sciences, University of Exeter, Penryn, Cornwall, UK;Station d’Ecologie Expérimentale du CNRS, USR 2936, 09200, Moulis, France;Evolutionary Morphology of Vertebrates, Ghent University, B-9000, Ghent, Belgium;UMR 7179 MECADEV, C.N.R.S/M.N.H.N., Département Adaptations du Vivant, 55 Rue Buffon, 75005, Paris, France;

关键词: Anura;    Limb;    Muscle;    Myosin;    RNA-sequencing;    Stamina;   

DOI  :  10.1186/s12864-021-07517-1

来源: Springer

【 摘 要 】

BackgroundVariation in locomotor capacity among animals often reflects adaptations to different environments. Despite evidence that physical performance is heritable, the molecular basis of locomotor performance and performance trade-offs remains poorly understood. In this study we identify the genes, signaling pathways, and regulatory processes possibly responsible for the trade-off between burst performance and endurance observed in Xenopus allofraseri, using a transcriptomic approach.ResultsWe obtained a total of about 121 million paired-end reads from Illumina RNA sequencing and analyzed 218,541 transcripts obtained from a de novo assembly. We identified 109 transcripts with a significant differential expression between endurant and burst performant individuals (FDR ≤ 0.05 and logFC ≥2), and blast searches resulted in 103 protein-coding genes. We found major differences between endurant and burst-performant individuals in the expression of genes involved in the polymerization and ATPase activity of actin filaments, cellular trafficking, proteoglycans and extracellular proteins secreted, lipid metabolism, mitochondrial activity and regulators of signaling cascades. Remarkably, we revealed transcript isoforms of key genes with functions in metabolism, apoptosis, nuclear export and as a transcriptional corepressor, expressed in either burst-performant or endurant individuals. Lastly, we find two up-regulated transcripts in burst-performant individuals that correspond to the expression of myosin-binding protein C fast-type (mybpc2). This suggests the presence of mybpc2 homoeologs and may have been favored by selection to permit fast and powerful locomotion.ConclusionThese results suggest that the differential expression of genes belonging to the pathways of calcium signaling, endoplasmic reticulum stress responses and striated muscle contraction, in addition to the use of alternative splicing and effectors of cellular activity underlie locomotor performance trade-offs. Ultimately, our transcriptomic analysis offers new perspectives for future analyses of the role of single nucleotide variants, homoeology and alternative splicing in the evolution of locomotor performance trade-offs.

【 授权许可】

CC BY