Lentinan administration alleviates diarrhea of rotavirus-infected weaned pigs via regulating intestinal immunity

Journal of Animal Science and Biotechnology

Erik Eckhardt¹  Jun He²  Jianping Wang²  Xiangqi Fan²  Haiyan Hu²  Bing Yu²  Xiangbing Mao²  Daiwen Chen²  Jie Yu²  Yuheng Luo²  Junqiu Luo²  Hui Yan² 

[1] Adisseo SAS, Center of Excellence and Research in Nutrition, 03600, Malicorne, France;Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Key laboratory of Animal Disease-resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Key laboratory of Animal Disease-resistant Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Gongpinghuimin Road, Wenjiang District, 611130, Chengdu, Sichuan Province, People’s Republic of China;

关键词: Gut immunity;    IPEC-J2 cells;    Lentinan;    Rotavirus;    Weaned pigs;   

DOI  :  10.1186/s40104-021-00562-6

来源: Springer

【 摘 要 】

BackgroundLentinan (LNT) may regulate many important physiological functions of human and animals. This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus (RV)-challenged weaned pigs.MethodsTwenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d (n = 14). RV infection was executed on d 15. After extracting polysaccharides from LNT product, its major monosaccharides were analyzed. Then, LNT polysaccharide was used to administrate RV-infected IPEC-J2 cells.ResultsDietary LNT supplementation supported normal function of piglets even when infected with RV, as reflected by reduced growth performance loss and diarrhea prevalence, and maintained gut immunity (P < 0.05). The polysaccharide was isolated from LNT product, which molecular weight was 5303 Da, and major monosaccharides included glucose, arabinose and galactose. In RV-infected IPEC-J2 cells, this polysaccharide significantly increased cell viability (P < 0.05), and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides (P < 0.05).ConclusionThose results suggest that LNT administration increases the piglets’ resistance to RV-induced stress, likely by supporting intestinal immunity.

【 授权许可】

CC BY