Characterization of hyaluronan-coated extracellular vesicles in synovial fluid of patients with osteoarthritis and rheumatoid arthritis

BMC Musculoskeletal Disorders

Antti Joukainen¹  Heikki Kröger¹  Tommi Kääriäinen¹  Janne Capra²  Kirsi Rilla³  Tommi Paakkonen³  Johanna Matilainen³  Sanna Oikari³  Anne-Mari Mustonen⁴  Petteri Nieminen⁴  Petri Lehenkari⁵ 

[1] Department of Orthopaedics, Traumatology and Hand Surgery, Kuopio University Hospital, P.O. Box 100, FI-70029, Kuopio, KYS, Finland;Faculty of Health Sciences, School of Medicine, Institute of Biomedicine, Cell and Tissue Imaging Unit, University of Eastern Finland, P.O. Box 1627, FI-70211, Kuopio, Finland;Faculty of Health Sciences, School of Medicine, Institute of Biomedicine, University of Eastern Finland, P.O. Box 1627, FI-70211, Kuopio, Finland;Faculty of Health Sciences, School of Medicine, Institute of Biomedicine, University of Eastern Finland, P.O. Box 1627, FI-70211, Kuopio, Finland;Faculty of Science and Forestry, Department of Environmental and Biological Sciences, University of Eastern Finland, P.O. Box 111, FI-80101, Joensuu, Finland;Faculty of Medicine, Cancer and Translational Medicine Research Unit, University of Oulu, P.O. Box 5000, FI-90014, Oulu, Finland;Department of Surgery and Medical Research Center, Oulu University Hospital, P.O. Box 21, FI-90029, Oulu, OYS, Finland;

关键词: Extracellular vesicles;    Hyaluronan;    Osteoarthritis;    Rheumatoid arthritis;    Synovial fluid;   

DOI  :  10.1186/s12891-021-04115-w

来源: Springer

【 摘 要 】

BackgroundHyaluronic acid (HA) is the major extracellular matrix glycosaminoglycan with a reduced synovial fluid (SF) concentration in arthropathies. Cell-derived extracellular vesicles (EV) have also been proposed to contribute to pathogenesis in joint diseases. It has recently been shown that human SF contains HA-coated EV (HA–EV), but their concentration and function in joint pathologies remain unknown.MethodsThe aim of the present study was to develop an applicable method based on confocal laser scanning microscopy (CLSM) and image analysis for the quantification of EV, HA-particles, and HA–EV in the SF of the human knee joint. Samples were collected during total knee replacement surgery from patients with end-stage rheumatoid arthritis (RA, n = 8) and osteoarthritis (OA, n = 8), or during diagnostic/therapeutic arthroscopy unrelated to OA/RA (control, n = 7). To characterize and quantify EV, HA-particles, and HA–EV, SF was double-stained with plasma membrane and HA probes and visualized by CLSM. Comparisons between the patient groups were performed with the Kruskal–Wallis analysis of variance.ResultsThe size distribution of EV and HA-particles was mostly similar in the study groups. Approximately 66% of EV fluorescence was co-localized with HA verifying that a significant proportion of EV carry HA. The study groups were clearly separated by the discriminant analysis based on the CLSM data. The intensities of EV and HA-particle fluorescences were lower in the RA than in the control and OA groups.ConclusionsCLSM analysis offers a useful tool to assess HA–EV in SF samples. The altered EV and HA intensities in the RA SF could have possible implications for diagnostics and therapy.

【 授权许可】

CC BY