Three-dimensional construction of micrometer level in rat stomach by synchrotron radiation

BioMedical Engineering OnLine

Jingdong Xu¹  Chenchen Gao¹  Xuehong Tong²  Shizhen Yuan³  Qiang Tao³ 

[1] Department of Physiology and Pathophysiology, School of Basic Medicine, Capital Medical University, 100069, Beijing, China;Experimental Centre for Basic Medical Teaching, School of Basic Medicine, Capital Medical University, 100069, Beijing, China;School of Biomedical Engineering, Capital Medical University, 100069, Beijing, China;

关键词: Synchrotron radiation phase-contrast imaging;    3-dimensional gastric structure images;    Different stages;   

DOI  :  10.1186/s12938-021-00866-8

来源: Springer

【 摘 要 】

BackgroundThe structural changes of gastric mucosa are considered as an important window of early gastric lesions. This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using micrometer level X-ray in-line phase-contrast imaging (XILPCI) technique on synchrotron radiation facility. The aim of the study was to demonstrate that the imaging technique is an appropriate method for micron imaging of the gastric structures.MethodsThe stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats stomachs were recorded by an XILPCI charge coupled device (CCD) at 9-μm image resolution.ResultsThe X-ray in-line phase-contrast images of the different stages of rats’ gastric specimens clearly showed the gastric architectures and the details of the gastro-duodenal region. 3-dimensional (3D) stomach anatomical structure images were reconstruction.ConclusionThe reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future.

【 授权许可】

CC BY