Numaswitch: an efficient high-titer expression platform to produce peptides and small proteins

AMB Express

Lutz Schmitt¹  Karl Erich Jaeger²  Julia Schneider³  Hilke Wobst³  Janpeter Stock³  Thomas Kalthoff³  Andreas Uhde³  Felix S. Schöpf³  Christian Schwarz³  Florian Tieves³  Jóse D. Montoya Solano³  Thomas Rohr⁴  Bach-Ngan Nguyen⁵ 

[1] Institute of Biochemistry, Heinrich-Heine University, 40225, Düsseldorf, Germany;Institute of Molecular Enzyme Technology, Heinrich Heine University, Düsseldorf, Germany;Institute of Bio- and Geosciences IGB-1: Biotechnology, Research Center Jülich, 52426, Jülich, Germany;NUMAFERM GmbH, Merowingerplatz 1a, 40225, Düsseldorf, Germany;NUMAFERM GmbH, Merowingerplatz 1a, 40225, Düsseldorf, Germany;Department of Applied Synthetic Chemistry, TU Wien, 2060, Vienna, Austria;NUMAFERM GmbH, Merowingerplatz 1a, 40225, Düsseldorf, Germany;Institute of Biochemistry, Heinrich-Heine University, 40225, Düsseldorf, Germany;

关键词: Numaswitch;    Switchtag;    Recombinant peptides;    High-titer peptide expression platform;    Teriparatide;    PTH(1-34);    PTH(1-84);    Liraglutide;    Semaglutide;    Active pharmaceutical ingredients;   

DOI  :  10.1186/s13568-021-01204-w

来源: Springer

【 摘 要 】

The production of peptides as active pharmaceutical ingredients (APIs) by recombinant technologies is of emerging interest. A reliable production platform, however, is still missing due the inherent characteristics of peptides such as proteolytic sensitivity, aggregation and cytotoxicity. We have developed a new technology named Numaswitch solving present limitations. Numaswitch was successfully employed for the production of diverse peptides and small proteins varying in length, physicochemical and functional characteristics, including Teriparatide, Linaclotide, human β-amyloid and Serum amyloid A3. Additionally, the potential of Numaswitch for a cost-efficient commercial production is demonstrated yielding > 2 g Teriparatide per liter fermentation broth in a quality meeting API standard.

【 授权许可】

CC BY