New Brucella variant isolated from Croatian cattle

BMC Veterinary Research

Irena Reil¹  Sanja Duvnjak¹  Maja Zdelar-Tuk¹  Silvio Spicic¹  Claire Ponsart²  Luca Freddi²  Guillaume Girault²  Martina Rubin³  Vesna Rukavina⁴  Rene S. Hendriksen⁵  Pimlapas Leekitcharoenphon⁵ 

[1]Department of Bacteriology and Parasitology, Laboratory for Bacterial Zoonosis and Molecular Diagnostics of Bacterial Diseases, Croatian Veterinary Institute, Savska street 143, Zagreb, Croatia

[2]French Agency for Food, Environmental & Occupational Health Safety (ANSES), Bacterial Zoonoses Unit - Animal Health Laboratory, National & OIE/FAO Animal Brucellosis Reference Laboratory, EU Reference Laboratory for Brucellosis, 14 rue Pierre et Marie Curie, Maisons-Alfort, Cedex, France

[3]Ministry of Agriculture, Veterinary and Food Safety Directorate, Department for Veterinary Epidemiology, Planinska 2a, 10000, Zagreb, Croatia

[4]Ministry of Agriculture, Veterinary and Food Safety Directorate, Veterinary Office Sisak, Branch Office Glina, Trg bana Josipa Jelačića 2, 44 400, Glina, Croatia

[5]Technical University of Denmark, National Food Institute, Research Group for Genomic Epidemiology, Kemitorvet, Building 204, 2800, Kgs. Lyngby, Denmark

关键词: Brucella melitensis;    Variant;    Cattle;    Eradication;    Croatia;   

DOI  :  10.1186/s12917-021-02833-w

来源: Springer

【 摘 要 】

BackgroundA novel Brucella strain closely related to Brucella (B.) melitensis biovar (bv) 3 was found in Croatian cattle during testing within a brucellosis eradication programme.Case presentationStandardised serological, brucellin skin test, bacteriological and molecular diagnostic screening for Brucella infection led to positive detection in one dairy cattle herd. Three isolates from that herd were identified to species level using the Bruce ladder method. Initially, two strains were typed as B. melitensis and one as B. abortus, but multiplex PCR based on IS711 and the Suis ladder showed that all of them to belong to B. melitensis, and the combination of whole-genome and multi-locus sequencing as well as Multi-Locus Variable numbers of tandem repeats Analysis (MLVA) highlighted a strong proximity within the phylogenetic branch of B. melitensis strains previously isolated from Croatia, Albania, Kosovo and Bosnia and Herzegovina. Two isolates were determined to be B. melitensis bv. 3, while the third showed a unique phylogenetic profile, growth profile on dyes and bacteriophage typing results. This isolate contained the 609-bp omp31 sequence, but not the 723-bp omp31 sequence present in the two isolates of B. melitensis bv. 3.ConclusionsIdentification of a novel Brucella variant in this geographic region is predictable given the historic endemicity of brucellosis. The emergence of a new variant may reflect a combination of high prevalence among domestic ruminants and humans as well as weak eradication strategies. The zoonotic potential, reservoirs and transmission pathways of this and other Brucella variants should be explored.

【 授权许可】

CC BY