RETRACTED ARTICLE: lncRNA TINCR sponges miR-214-5p to upregulate ROCK1 in hepatocellular carcinoma

BMC Medical Genetics

Min Hu¹  Yaowu Han¹  Lin Ye²  Ying Zhang³  Yuanfeng Zhou³ 

[1] Department of Pathology, Anhui University of Chinese Medicine, Hefei, 1 Qianjiang Road, 230012, Hefei, Anhui province, China;Department of Psychology, Anhui University of Chinese Medicine, 230012, Hefei, Anhui, China;Graduate School, Anhui University of Chinese Medicine, 230038, Hefei, Anhui, China;

关键词: Hepatocellular carcinoma;    lncRNA TINCR;    miR-214-5p;    ROCK1;   

DOI  :  10.1186/s12881-019-0940-6

来源: Springer

【 摘 要 】

BackgroundOur preliminary bioinformatics analysis showed that lncRNA TINCR may absorb miR-214-5p by serving is sponge, while miR-214-5p targets ROCK1. This study aimed to investigate the interactions among these 3 factors in hepatocellular carcinoma (HCC).MethodsExpression of TINCR, ROCK1 and miR-214-5p in HCC and non-tumor tissues was detected by performing qPCR. The correlations among TINCR, ROCK1 and miR-214-5p in HCC tissues were analyzed by performing linear regression. Overexpression experiments were performed to analyze gene interactions. Cell proliferation was analyzed by CCK-8 assay.ResultsWe found that TINCR and ROCK1 were upregulated, while miR-214-5p was downregulated in HCC. TINCR and ROCK1 were positively correlated, while TINCR and miR-214-5p were not significantly correlated. In HCC cells, TINCR overexpression is followed by ROCK1 overexpression, while miR-214-5p overexpression induced the downregulation of ROCK1. In addition, TINCR and miR-214-5p did not affect the expression of each other. TINCR and ROCK1 overexpression led to increased rate of cancer cell proliferation, while miR-214-5p played an opposite role and reduced the effects of TINCR overexpression. Therefore, TINCR sponges miR-214-5p to upregulate ROCK1 in HCC, thereby promoting cancer cell invasion and migration.

【 授权许可】

CC BY