Isolation and characterization of antagonistic Paenibacillus polymyxa HX-140 and its biocontrol potential against Fusarium wilt of cucumber seedlings

BMC Microbiology

Ji-lie Li¹  Ai-rong Shen²  Liang-bin Zeng³  Jian-ping Xu³  Yang Zhai³  Tai-meng Tan⁴  Xie-bin Yang⁴  Jiu-xiang Zhu⁴  Lin Wei⁵ 

[1] Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, 410004, Changsha, China;Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, 410004, Changsha, China;Hunan Academy of Forestry, 410004, Changsha, China;Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, No.348 Xianjiahu West Road, 410205, Changsha, Hunan, China;Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, No.348 Xianjiahu West Road, 410205, Changsha, Hunan, China;Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, 410004, Changsha, China;Institute of Plant Protection, Hunan Academy of Agricultural Sciences, No.726 Yuanda 2nd Road, 410125, Changsha, Hunan, China;

关键词: Fusarium wilt;    Cucumber;    Paenibacillus polymyxa;    Antagonistic bacterium;    Biocontrol mechanism;   

DOI  :  10.1186/s12866-021-02131-3

来源: Springer

【 摘 要 】

ObjectiveThe aim of this study is to evaluate the efficacy of the strain Paenibacillus polymyxa HX-140, isolated from the rhizosphere soil of rape, to control Fusarium wilt of cucumber seedlings caused by Fusarium oxysporum f. sp. cucumerinum.ResultsStrain HX-140 was able to produce protease, cellulase, β-1,3-glucanase and antifungal volatile organic compounds. An in vitro dual culture test showed that strain HX-140 exhibited broad spectrum antifungal activity against soil-borne plant pathogenic fungi. Strain HX-140 also reduced the infection of Fusarium wilt of cucumber seedlings by 55.6% in a greenhouse pot experiment. A field plot experiment confirmed the biocontrol effects and further revealed that antifungal activity was positively correlated with inoculum size by the root-irrigation method. Here, inoculums at 106 107 and 108 cfu/mL of HX-140 bacterial suspension reduced the incidence of Fusarium wilt of cucumber seedling by 19.5, 41.1, and 50.9%, respectively.ConclusionsTaken together, our results suggest that P. polymyxa HX-140 has significant potential in the control of Fusarium wilt and possibly other fungal diseases of cucumber.

【 授权许可】

CC BY