Fluralaner as a novel treatment for sarcoptic mange in the bare-nosed wombat (Vombatus ursinus): safety, pharmacokinetics, efficacy and practicable use

Parasites & Vectors

Ruth Pye¹  Alexandre Kreiss¹  Roz Holme²  David Nichols³  Scott Carver⁴  Michael Charleston⁴  Alynn Martin⁴  Vicky Wilkinson⁴  Christina Næsborg-Nielsen⁴  Shane A. Richards⁴  Elizabeth Browne⁴  David Phalen⁵  Kotaro Takano⁶  Kate Mounsey⁶ 

[1] Bonorong Wildlife Sanctuary, 593 Briggs Rd, Brighton, Tasmania, Australia;Cedar Creek Wombat Rescue Inc, PO Box 538, Cessnock, NSW, Australia;Central Science Laboratory, University of Tasmania, Private Bag 74, Hobart, Tasmania, Australia;School of Natural Sciences, University of Tasmania, Private Bag 55, Hobart, Tasmania, Australia;The University of Sydney, C01A, JI Shute, Camden, Sydney, NSW, Australia;The University of the Sunshine Coast, 90 Sippy Downs Dr, Sippy Downs, QLD, Australia;

关键词: Fluralaner;    Sarcoptic mange;    Sarcoptes scabiei;    Bare-nosed wombat;    Safety;    Pharmacokinetics;    Efficacy;   

DOI  :  10.1186/s13071-020-04500-9

来源: Springer

【 摘 要 】

BackgroundSarcoptic mange causes significant animal welfare and occasional conservation concerns for bare-nosed wombats (Vombatus ursinus) throughout their range. To date, in situ chemotherapeutic interventions have involved macrocytic lactones, but their short duration of action and need for frequent re-administration has limited treatment success. Fluralaner (Bravecto®; MSD Animal Health), a novel isoxazoline class ectoparasiticide, has several advantageous properties that may overcome such limitations.MethodsFluralaner was administered topically at 25 mg/kg (n = 5) and 85 mg/kg (n = 2) to healthy captive bare-nosed wombats. Safety was assessed over 12 weeks by clinical observation and monitoring of haematological and biochemical parameters. Fluralaner plasma pharmacokinetics were quantified using ultra-performance liquid chromatography and tandem mass spectrometry. Efficacy was evaluated through clinical assessment of response to treatment, including mange and body condition scoring, for 15 weeks after topical administration of 25 mg/kg fluralaner to sarcoptic mange-affected wild bare-nosed wombats (n = 3). Duration of action was determined through analysis of pharmacokinetic parameters and visual inspection of study subjects for ticks during the monitoring period. Methods for diluting fluralaner to enable ‘pour-on’ application were compared, and an economic and treatment effort analysis of fluralaner relative to moxidectin was undertaken.ResultsNo deleterious health impacts were detected following fluralaner administration. Fluralaner was absorbed and remained quantifiable in plasma throughout the monitoring period. For the 25 mg/kg and 85 mg/kg treatment groups, the respective means for maximum recorded plasma concentrations (Cmax) were 6.2 and 16.4 ng/ml; for maximum recorded times to Cmax, 3.0 and 37.5 days; and for plasma elimination half-lives, 40.1 and 166.5 days. Clinical resolution of sarcoptic mange was observed in all study animals within 3–4 weeks of treatment, and all wombats remained tick-free for 15 weeks. A suitable product for diluting fluralaner into a ‘pour-on’ was found. Treatment costs were competitive, and predicted treatment effort was substantially lower relative to moxidectin.ConclusionsFluralaner appears to be a safe and efficacious treatment for sarcoptic mange in the bare-nosed wombat, with a single dose lasting over 1–3 months. It has economic and treatment-effort-related advantages over moxidectin, the most commonly used alternative. We recommend a dose of 25 mg/kg fluralaner and, based on the conservative assumption that at least 50% of a dose makes dermal contact, Bravecto Spot-On for Large Dogs as the most appropriate formulation for adult bare-nosed wombats.

【 授权许可】

CC BY