Single-cell map of diverse immune phenotypes in the acute myeloid leukemia microenvironment

Biomarker Research

Rong Guo¹  Haili Pang¹  Zhongxing Jiang¹  Xinsheng Xie¹  Fengcai Gao¹  Yinyin Zhang¹  Delong Liu¹  Haizhou Xing¹  Wei Li¹  Chunyan Liang¹  Rongqun Guo¹  Fujiao Cao¹  Yingmei Li¹  Yongping Song¹  Mengdie Lü²  Chunyan Du³  Guanghua Wu⁴  Yadan Li⁵  Tianxin Lyu⁵ 

[1] Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China;Joint National Laboratory for Antibody Drug Engineering, Key Laboratory of Cellular and Molecular Immunology of Henan Province, Institute of Translational Medicine, School of Basic Medicine, Henan University, Kaifeng, Henan, China;Laboratory Animal Center, School of Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China;The Academy of Medical Science, College of Medical, Zhengzhou University, Zhengzhou, Henan, China;The Academy of Medical Science, College of Medical, Zhengzhou University, Zhengzhou, Henan, China;The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, China;

关键词: Acute myeloid leukemia;    Microenvironment;    Single-cell RNA sequencing;    Immune phenotypes;    Bone marrow;    Immune cells;    Myeloid cells;    T lymphocytes;   

DOI  :  10.1186/s40364-021-00265-0

来源: Springer

【 摘 要 】

BackgroundKnowledge of immune cell phenotypes, function, and developmental trajectory in acute myeloid leukemia (AML) microenvironment is essential for understanding mechanisms of evading immune surveillance and immunotherapy response of targeting special microenvironment components.MethodsUsing a single-cell RNA sequencing (scRNA-seq) dataset, we analyzed the immune cell phenotypes, function, and developmental trajectory of bone marrow (BM) samples from 16 AML patients and 4 healthy donors, but not AML blasts.ResultsWe observed a significant difference between normal and AML BM immune cells. Here, we defined the diversity of dendritic cells (DC) and macrophages in different AML patients. We also identified several unique immune cell types including T helper cell 17 (TH17)-like intermediate population, cytotoxic CD4+ T subset, T cell: erythrocyte complexes, activated regulatory T cells (Treg), and CD8+ memory-like subset. Emerging AML cells remodels the BM immune microenvironment powerfully, leads to immunosuppression by accumulating exhausted/dysfunctional immune effectors, expending immune-activated types, and promoting the formation of suppressive subsets.ConclusionOur results provide a comprehensive AML BM immune cell census, which can help to select pinpoint targeted drug and predict efficacy of immunotherapy.

【 授权许可】

CC BY