Characterization of the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds by SR-FTIR

BMC Musculoskeletal Disorders

Hongbin Lu¹  Muzhi Li¹  Yan Xu¹  Yang Chen¹  Qiang Shi¹  Jun Liu²  Can Chen³  Jianzhong Hu⁴ 

[1] Department of Sports Medicine, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, China;Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, 410008, Changsha, Hunan, China;Xiangya Hospital-International Chinese Musculoskeletal Research Society Sports Medicine Research Centre, 410008, Changsha, Hunan, China;Department of limbs (foot and hand) microsurgery, Affiliated Chenzhou No.1 People’s Hospital, Southern Medical University, 423000, Chenzhou, Hunan, China;Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, 410008, Changsha, Hunan, China;Xiangya Hospital-International Chinese Musculoskeletal Research Society Sports Medicine Research Centre, 410008, Changsha, Hunan, China;Department of Orthopedics, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, China;Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, 410008, Changsha, Hunan, China;Xiangya Hospital-International Chinese Musculoskeletal Research Society Sports Medicine Research Centre, 410008, Changsha, Hunan, China;Department of Spine Surgery, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, China;

关键词: SR-FTIR;    Decellularized book-shaped enthesis scaffolds;    Bone-tendon interface;    Rabbit rotator cuff;   

DOI  :  10.1186/s12891-021-04106-x

来源: Springer

【 摘 要 】

BackgroundBone-tendon interface (enthesis) plays a pivotal role in relaxing load transfer between otherwise structurally and functionally distinct tissue types. Currently, decellularized extracellular matrix (DEM) from enthesis provide a natural three-dimensional scaffold with tissue-specific orientations of extracellular matrix molecules for enthesis regeneration, however, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff by SR-FTIR have not been reported.MethodsNative enthesis tissues (NET) harvested from rabbit rotator cuff were sectioned into cuboid (about 30 mm × 1.2 mm × 10 mm) for decalcification. The decellularized book-shaped enthesis scaffolds and intrinsic ultrastructure were evaluated by histological staining and scanning electron microscopy (SEM), respectively. The distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were also measured innovatively by SR-FTIR.ResultsThe decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were successfully obtained. Histomorphology and SEM evaluated the effect of decellularization and the structure of extracellular matrix during decellularization. After mechanical testing, the failure load in the NET group showed significantly higher than that in the DEM group (P < 0.05). Meanwhile, the stiffness of the DEM group was significantly lower than the NET group. Furthermore, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds were decreased obviously after decellularization by SR-FTIR quantitative analysis.ConclusionSR-FTIR was applied innovatively to characterize the histological morphology of native enthesis tissues from rabbit rotator cuff. Moreover, this technology can be applied for quantitative mapping of the distribution of collagen and PGs content in the decellularized book-shaped enthesis scaffolds.

【 授权许可】

CC BY