XIST and RPS4Y1 long non-coding RNA transcriptome as sex biomarkers in different body fluids

Egyptian Journal of Forensic Sciences

Fatma Mohamed Hassan¹  Heba Abdo Abdel Razik¹  Miriam Safwat Wadie²  Dina Sabry Abdelfattah² 

[1] Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University, Kasr Alainy Street, 11562, Cairo, Egypt;Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt;

关键词: Sex identification;    Body fluids;    Long non-coding ribonucleic acid;    X-inactive specific transcript;    Ribosomal protein S4, Y-linked 1;   

DOI  :  10.1186/s41935-019-0122-x

来源: Springer

【 摘 要 】

Background and objectivesSex determination of an individual based on biological fluid evidence is a critical issue in forensic science. RNA has proved valuable in the identification of body fluids and the estimation of stain age. However, it still could not provide sufficient information about their donor. This study aimed to evaluate the potential use of the long non-coding XIST and RPS4Y1 markers in the identification of sex from different body fluids.MethodsSaliva, semen, and peripheral and menstrual blood samples were obtained from apparently healthy volunteers. The expression of XIST and RPS4Y1 was assessed in these samples using real-time RT-PCR.ResultsXIST was detected in female saliva and peripheral and menstrual blood, while RPS4Y1 was detected in male blood and semen.ConclusionXIST and RPS4Y1 could be sex differentiation biomarkers in various body fluids.

【 授权许可】

CC BY