Functional emergence of a column-like architecture in layer 5 of mouse somatosensory cortex in vivo

The journal of physiological sciences

article

Kyo Koizumi¹  Masatoshi Inoue²  Srikanta Chowdhury³  Haruhiko Bito²  Akihiro Yamanaka³  Toru Ishizuka¹  Hiromu Yawo¹ 

[1]Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences

[2]Department of Neurochemistry, Graduate School of Medicine, University of Tokyo

[3]Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency

[4]Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University

[5]Center for Neuroscience, Tohoku University Graduate School of Medicine

关键词: Optogenetics;    Ca 2+ imaging;    Two-photon microscopy;    Self-organization;    Column;    Reciprocal inhibition;   

DOI  :  10.1007/s12576-018-0618-4

来源: Springer

【 摘 要 】

To investigate how the functional architecture is organized in layer 5 (L5) of the somatosensory cortex of a mouse in vivo, the input–output relationship was investigated using an all-optical approach. The neural activity in L5 was optically recorded using a Ca2+ sensor, R-CaMP2, through a microprism inserted in the cortex under two-photon microscopy, while the L5 was regionally excited using optogenetics. The excitability was spread around the blue-light irradiated region, but the horizontal propagation was limited to within a certain distance (λ < 130 μm from the center of the illumination spot). When two regions were photostimulated with a short interval, the excitability of each cluster was reduced. Therefore, a column-like architecture had functionally emerged with reciprocal inhibition through a minimal number of synaptic relays. This could generate a synchronous output from a region of L5 with simultaneous enhancement of the signal-to-noise ratio by silencing of the neighboring regions.

【 授权许可】

Unknown