Cytoskeleton disruption affects Kv2.1 channel function and its modulation by PIP 2

The journal of physiological sciences

article

Mayra Delgado-Ramírez¹  Aldo A. Rodríguez-Menchaca¹ 

[1] Universidad Autónoma de San Luis Potosí

关键词: Kv channels;    Cytoskeleton;    Latrunculin B;    Phalloidin;    PIP 2;    Patch clamp;   

DOI  :  10.1007/s12576-019-00671-y

来源: Springer

【 摘 要 】

Voltage-gated potassium channels are expressed in a wide variety of excitable and non-excitable cells and regulate numerous cellular functions. The activity of ion channels can be modulated by direct interaction or/and functional coupling with other proteins including auxiliary subunits, scaffold proteins and the cytoskeleton. Here, we evaluated the influence of the actin-based cytoskeleton on the Kv2.1 channel using pharmacological and electrophysiological methods. We found that disruption of the actin-based cytoskeleton by latrunculin B resulted in the regulation of the Kv2.1 inactivation mechanism; it shifted the voltage of half-maximal inactivation toward negative potentials by approximately 15 mV, accelerated the rate of closed-state inactivation, and delayed the recovery rate from inactivation. The actin cytoskeleton stabilizing agent phalloidin prevented the hyperpolarizing shift in the half-maximal inactivation potential when co-applied with latrunculin B. Additionally, PIP2 depletion (a strategy that regulates Kv2.1 inactivation) after cytoskeleton disruption does not regulate further the inactivation of Kv2.1, which suggests that both factors could be regulating the Kv2.1 channel by a common mechanism. In summary, our results suggest a role for the actin-based cytoskeleton in regulating Kv2.1 channels.

【 授权许可】

Unknown