LAMP Assay: Could it be a Boon for the Molecular Diagnosis of COVID-19?

Indian journal of clinical biochemistry

article

P. Buella Parivallal¹  M. K. Prasanna Kumar³  K. C. Vasanthakumar¹  Fathima F. Thavuroollah¹  Yamuna Manoharan¹  Sundaram Muthu¹ 

[1] Winomicx Molecular Diagnostics and Research Pvt Ltd, Golden Jubilee Biotech Park for Women Society;Dendrite Laboratories Pvt Ltd;Department of Plant Pathology, University of Agricultural Sciences

关键词: LAMP assay COVID-19 RT-PCR Isothermal amplification Low cost;   

DOI  :  10.1007/s12291-020-00915-4

来源: Springer

【 摘 要 】

Severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) has become a major threat for mankind and poses a great challenge to global economy. On 31st December 2019, the World Health Organization (WHO) was informed about a cluster of pneumonia cases in Wuhan, China [1]. Subsequent investigations identified a completely unique sequence of coronavirus which differed from existing type SARS-CoV known as SARS-CoV-2. Initially, it was observed within the Wuhan province of China but later spread fast to rest of the world, which forced WHO to declare COVID-19 as pandemic. So far there is a total of 10,302,867 confirmed cases, with 505,518 global deaths, while 5,558,161 are recovered cases. In India, a total of 5,68,473 confirmed cases out of which 2,15,838 of active cases and 3,35,656 of recovered cases also 16,919 of the death cases are reported till date. The SARS-CoV-2 is a single-stranded, positive-sense RNA virus [2] with its complete genetic sequence been decoded and made available to the Global Initiative on Sharing All Influenza Data (GISAID) platform on January 10, 2020. This provided an opportunity for several R&D companies and research institutes to design specific primers targeting COVID-19 which further lead to the development of RTPCR diagnostic kits (NCBI Reference Sequence: NC_045512.2).

【 授权许可】

Unknown