Evaluation of the Expression Level and Hormone Receptor Association of miR-126 in Breast Cancer

Indian journal of clinical biochemistry

article

Maedeh Rouigari¹  Moein Dehbashi²  Hossein Tabatabaeian²  Kamran Ghaedi⁴  Parisa Mohammadynejad¹  Mansoureh Azadeh⁶ 

[1] Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University;Division of Genetics, Department of Biology, Faculty of Sciences, University of Isfahan;Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore;Division of Cellular and Molecular Biology, Department of Biology, Faculty of Sciences, University of Isfahan;Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology;Zist Fanavari Novin Biotechnology Institute

关键词: miR-126 Estrogen receptor Progesterone receptor HER-2 Breast cancer;   

DOI  :  10.1007/s12291-018-0766-6

来源: Springer

【 摘 要 】

Breast cancer is a major cause of cancer-related death in women worldwide. miRNAs are new players of breast tumorigenesis, used as diagnostic and prognostic biomarkers. Among various miRNAs, miR-126 has been proposed to have a tumor suppressive role in HER2 positive cancer. However, to have a better understanding of its role, further validation is required. The aim of this study was evaluating miR-126 expression level in breast cancer tissues and investigating its potential association with HER2, estrogen and progesterone receptors. miR-126 expression level was measured in 108 specimens including 78 malignant and 30 normal samples using RT-qPCR. The outcome was statistically analyzed. In silico studies were performed to find the potential mechanism of action, through which miR-126 imposes its function. Down-regulation of miR-126 was observed in tumor samples, as compared to the matched normal tissues. Down-regulation of miR-126 was also associated significantly with the absence of estrogen receptor in malignant samples. No association between miR-126 expression and HER2 status was observed. Our in silico analyses showed the possible role of Crk, PI3K and Ras proto-oncogenes in breast cancer tumorigenesis. miR-126 is significantly down-regulated in breast cancer tissues. Statistically, it showed no correlation with HER2 positivity. However, the association between lower miR-126 and estrogen receptor negativity was observed.

【 授权许可】

Unknown