A Simple Method for Quantification of Five Urinary Porphyrins, Porphobilinogen and 5-Aminolevulinic Acid, Using Liquid Chromatography Tandem Mass Spectrometry

Indian journal of clinical biochemistry

article

Ozlem Dogan¹  Muhittin A. Serdar²  Koza Murat³  Cigdem Sonmez⁴  Emre İspir⁵  Mustafa Serteser²  Ibrahim Unsal² 

[1] Department of Medical Biochemistry, Ankara University Faculty of Medicine;Department of Medical Biochemistry, Acibadem University;Ataturk Chest Disease and Thoracic Surgery Education and Research Hospital;Department of Biochemistry, Dr Abdurrahman Yurtarslan Education and Research Hospital;Department of Biochemistry, Gulhane Military Medical Hospital

关键词: Porphyrins LC–MS ALA Porphobilinogen;   

DOI  :  10.1007/s12291-017-0716-8

来源: Springer

【 摘 要 】

Analysis of porphyrins and 5-aminolevulinic acid (ALA), porphobilinogen (PBG) in physiological liquids is required for diagnosis and follow-up of porphyrias. High performance liquid chromatography (HPLC) and liquid chromatography tandem mass spectrometry (LC–MS) methods with higher specificity and sensitivity have been developed. The major disadvantage of those methods is that they require longer extraction times due to their matrix effects. The present study suggests a simple, fast, sensitive, and specific assay for determination of Coproporphyrin, 5-carboxylporphyrin, 6-carboxylporphyrin, 7-carboxylporphyrin, Uroporphyrin I and ALA, PBG in urine sample by direct injection without sample pre-treatment using LC–MS. For the purposes of the present study LC–MS device was set to multiple reaction monitoring (MRM) and positive ion mode. Porphyrins and ALA, porphobilinogen were characterized by their MS/MS product ion, spectra. ALA, PBG and 5 porphyrins were detected simultaneously. Limit of detection for Coproporphyrin, 5-carboxylporphyrin, 6-carboxylporphyrin, 7-carboxylporphyrin, Uroporphyrin I were 2 nmol/L, where it was 5 μmol/L for ALA and 2 μmol/L for porphobilinogen. The present study suggests that the present method is very effective compared to many other available methods for it does not require pre-treatment, provides simultaneous results of ALA, PBG and 5 porphyrins quantitatively in a shorter span of time, and has suitable sensitivity and selectivity. LC–MS technique was used clinically for the determination of urine porphyrin levels.

【 授权许可】

Unknown