Characterization of Methacrylated Type-I Collagen as a Dynamic, Photoactive Hydrogel

Biointerphases

article

Ian D. Gaudet¹  David I. Shreiber¹ 

[1] Department of Biomedical Engineering, The State University of New Jersey

关键词: Storage Modulus;    Native Collagen;    Genipin;    Circular Dichroism Spectroscopy;    Free Amine;   

DOI  :  10.1007/s13758-012-0025-y

学科分类：基础医学

来源: SpringerOpen

【 摘 要 】

Type-I collagen is an attractive scaffold material for tissue engineering due to its ability to self-assemble into a fibrillar hydrogel, its innate support of tissue cells through bioactive adhesion sites, and its biodegradability. However, a lack of control of material properties has hampered its utility as a scaffold. We have modified collagen via the addition of methacrylate groups to create collagen methacrylamide (CMA) using a synthesis reaction that allows retention of fundamental characteristics of native collagen, including spontaneous fibrillar self-assembly and enzymatic biodegradability. This method allows for a rapid, five-fold increase in storage modulus upon irradiation with 365 nm light. Fibrillar diameter of CMA was not significantly different from native collagen. Collagenolytic degradability of uncrosslinked CMA was minimally reduced, while photocrosslinked CMA was significantly more resistant to degradation. Live/Dead staining demonstrated that a large majority (71%) of encapsulated mesenchymal stem cells remained viable 24 h after photocrosslinking, which further increased to 81% after 72 h. This material represents a novel platform for creating mechanically heterogeneous environments.

【 授权许可】

CC BY