ARGET–ATRP Synthesis and Characterization of PNIPAAm Brushes for Quantitative Cell Detachment Studies

Biointerphases

article

Phanindhar Shivapooja¹  Linnea K. Ista²  Heather E. Canavan²  Gabriel P. Lopez¹ 

[1] Department of Biomedical Engineering, Duke University;Department of Chemical and Nuclear Engineering, Center for Biomedical Engineering, University of New Mexico

关键词: Atom Transfer Radical Polymerization;    Lower Critical Solution Temperature;    Polymerization Time;    Polymer Brush;    Atom Transfer Radical Polymerization Initiator;   

DOI  :  10.1007/s13758-012-0032-z

学科分类：基础医学

来源: SpringerOpen

【 摘 要 】

Stimuli responsive (or “smart”) polymer brushes represent a non-toxic approach for achieving release of biofouling layers. Thermo-responsive poly(N-isopropylacrylamide) (PNIPAAm) polymer brushes have been shown to modulate bacterial adhesion and release through transition between temperatures above and below the lower critical solution temperature (LCST ~32 °C) of PNIPAAm in water. In this article, we describe a convenient method to synthesize grafted PNIPAAm brushes over large areas for biological studies using a relatively simple and rapid method which allows atom transfer radical polymerization (ATRP) in presence of air using the activator regenerated electron transfer (ARGET) mechanism. PNIPAAm brushes were characterized using X-ray photoelectron spectroscopy, time-of-flight secondary ion mass spectroscopy, Fourier transform infrared spectroscopy, ellipsometry, and contact angle measurements. Our studies demonstrate that uniform, high purity PNIPAAm brushes with controlled and high molecular weight can be easily produced over large areas using ARGET–ATRP. We also report the use of a spinning disk apparatus to systematically and quantitatively study the detachment profiles of bacteria from PNIPAAm surfaces under a range (0–400 dyne/cm2) of shear stresses.

【 授权许可】

CC BY