The bacterial communities of Alaskan mosses and their contributions to N2-fixation

Microbiome

Michelle C. Mack¹  Julia E. M. Stuart¹  Samantha N. Miller¹  Jose Miguel Ponciano²  Stuart F. McDaniel²  Hannah Holland-Moritz³  Noah Fierer⁴  Lily R. Lewis⁵ 

[1] Center for Ecosystem Science and Society and the Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ, USA;Department of Biology, University of Florida, Gainesville, FL, USA;Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, CO, USA;Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, CO, USA;Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, CO, USA;Provost’s Office, University of Florida, Gainesville, FL, USA;

关键词: Bryophytes;    Plant microbiome;    Phyllosphere;    Nitrogen fixation;   

DOI  :  10.1186/s40168-021-01001-4

来源: Springer

【 摘 要 】

BackgroundMosses in high-latitude ecosystems harbor diverse bacterial taxa, including N2-fixers which are key contributors to nitrogen dynamics in these systems. Yet the relative importance of moss host species, and environmental factors, in structuring these microbial communities and their N2-fixing potential remains unclear. We studied 26 boreal and tundra moss species across 24 sites in Alaska, USA, from 61 to 69° N. We used cultivation-independent approaches to characterize the variation in moss-associated bacterial communities as a function of host species identity and site characteristics. We also measured N2-fixation rates via 15N2 isotopic enrichment and identified potential N2-fixing bacteria using available literature and genomic information.ResultsHost species identity and host evolutionary history were both highly predictive of moss microbiome composition, highlighting strong phylogenetic coherence in these microbial communities. Although less important, light availability and temperature also influenced composition of the moss microbiome. Finally, we identified putative N2-fixing bacteria specific to some moss hosts, including potential N2-fixing bacteria outside well-studied cyanobacterial clades.ConclusionsThe strong effect of host identity on moss-associated bacterial communities demonstrates mosses’ utility for understanding plant-microbe interactions in non-leguminous systems. Our work also highlights the likely importance of novel bacterial taxa to N2-fixation in high-latitude ecosystems.D9s3_gqDR2-mjeknv8S5sZVideo Abstract

【 授权许可】

CC BY