Anterior cruciate ligament reconstruction in a rabbit model using a silk-collagen scaffold modified by hydroxyapatite at both ends: a histological and biomechanical study

Journal of Orthopaedic Surgery and Research

Danfeng Xu¹  Yafei Wang²  Ke Tian²  Fanggang Bi²  Junqi Liu³  Yangdi Chen⁴ 

[1] Department of Orthopedic Surgery, Shaoxing Central Hospital, NO.1 Huayu Road, 312000, Shaoxing, China;Department of Orthopedic Surgery, The First Affiliated Hospital of Zhengzhou University, NO.1 Jianshe East Road, 450001, Zhengzhou, China;Department of Radiation Oncology, The First Affiliated Hospital of Zhengzhou University, NO.1 Jianshe East Road, 450001, Zhengzhou, China;Henan University of Chinese Medicine, NO.156 Jinshui East Road, 450001, Zhengzhou, China;

关键词: Hydroxyapatite;    Anterior cruciate ligament reconstruction;    Tendon-bone healing;    Osteointegration;   

DOI  :  10.1186/s13018-021-02281-0

来源: Springer

【 摘 要 】

BackgroundTo investigate osteointegration at the graft-bone interface and the prevention of osteoarthritis after anterior cruciate ligament (ACL) reconstruction using a silk-collagen scaffold with both ends modified by hydroxyapatite (HA) in a rabbit model.MethodsThe HA/silk-collagen scaffold was fabricated using a degummed, knitted silk scaffold, collagen I matrix, and simulated body fluid (SBF). The HA/silk-collagen scaffold was rolled up to make a graft for replacing the native ACL in the experimental group (HA group), and the silk-collagen scaffold was used in the control (S group). All specimens were harvested at 16 weeks postoperatively to evaluate graft-bone healing and osteoarthritis prevention.ResultsHistological staining revealed the massive formation of more mature bone at the tendon-bone interface, and immunohistochemistry staining revealed more collagen I and osteocalcin deposition in the HA group than in the S group. Higher signals indicating more bone mineral formation were detected in the HA group than in the S group, which was consistent with the results of biomechanical testing. Better osteoarthritis prevention was also observed in the HA group, indicating a more stable knee joint in the HA group than in the S group.ConclusionThe HA/silk-collagen scaffold promotes osteointegration at the tendon-bone interface after ACL reconstruction and has great potential for clinical applications.

【 授权许可】

CC BY