Mesenchymal stem cell-derived conditioned medium protects vascular grafts of brain-dead rats against in vitro ischemia/reperfusion injury

Stem Cell Research & Therapy

Matthias Karck¹  Yuxing Guo¹  Sevil Korkmaz-Icöz¹  Paige Brlecic¹  Pengyu Zhou¹  Gábor Szabó²  Gábor Veres²  Mihály Ruppert³  Alex Ali Sayour³  Sivakkanan Loganathan⁴  Tamás Radovits⁵ 

[1] Department of Cardiac Surgery, Laboratory of Cardiac Surgery, University Hospital Heidelberg, INF 326, 69120, Heidelberg, Germany;Department of Cardiac Surgery, Laboratory of Cardiac Surgery, University Hospital Heidelberg, INF 326, 69120, Heidelberg, Germany;Department of Cardiac Surgery, University Hospital Halle (Saale), 06120, Halle, Germany;Department of Cardiac Surgery, Laboratory of Cardiac Surgery, University Hospital Heidelberg, INF 326, 69120, Heidelberg, Germany;Heart and Vascular Center, Semmelweis University, 1122, Budapest, Hungary;Department of Cardiac Surgery, University Hospital Halle (Saale), 06120, Halle, Germany;Heart and Vascular Center, Semmelweis University, 1122, Budapest, Hungary;

关键词: Ischemia/reperfusion;    Endothelial function;    Mesenchymal stem cells;    Conditioned medium;    Brain death;   

DOI  :  10.1186/s13287-021-02166-3

来源: Springer

【 摘 要 】

BackgroundBrain death (BD) has been suggested to induce coronary endothelial dysfunction. Ischemia/reperfusion (IR) injury during heart transplantation may lead to further damage of the endothelium. Previous studies have shown protective effects of conditioned medium (CM) from bone marrow-derived mesenchymal stem cells (MSCs) against IR injury. We hypothesized that physiological saline-supplemented CM protects BD rats’ vascular grafts from IR injury.MethodsThe CM from rat MSCs, used for conservation purposes, indicates the presence of 23 factors involved in apoptosis, inflammation, and oxidative stress. BD was induced by an intracranial-balloon. Controls were subjected to a sham operation. After 5.5 h, arterial pressures were measured in vivo. Aortic rings from BD rats were harvested and immediately mounted in organ bath chambers (BD group, n = 7) or preserved for 24 h in 4 °C saline-supplemented either with a vehicle (BD-IR group, n = 8) or CM (BD-IR+CM group, n = 8), prior to mounting. Vascular function was measured in vitro. Furthermore, immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR) have been performed.ResultsBD in donors was associated with significantly impaired hemodynamic parameters and higher immunoreactivity of aortic myeloperoxidase (MPO), nitrotyrosine, caspase-3, caspase-8, caspase-9, and caspase-12 compared to sham-operated rats. In organ bath experiments, impaired endothelium-dependent vasorelaxation to acetylcholine in the BD-IR group compared to BD rats was significantly improved by CM (maximum relaxation to acetylcholine: BD 81 ± 2% vs. BD-IR 50 ± 3% vs. BD-IR + CM 72 ± 2%, p < 0.05). Additionally, the preservation of BD-IR aortic rings with CM significantly lowered MPO, caspase-3, caspase-8, and caspase-9 immunoreactivity compared with the BD-IR group. Furthermore, increased mRNA expression of vascular cell adhesion molecule (VCAM)-1 and intercellular adhesion molecule (ICAM)-1 in the aortas from the BD-IR rats compared to BD group were significantly decreased by CM.ConclusionsThe preservation of BD rats’ vascular grafts with CM alleviates endothelial dysfunction following IR injury, in part, by reducing levels of inflammatory response and caspase-mediated apoptosis.

【 授权许可】

CC BY