Microbial yield from infectious tissues pretreated by various methods: an invitro study

BMC Musculoskeletal Disorders

Bin Yang¹  Lvheng Zhang²  Lixiong Nie²  Wenming Zhang²  Xurong Yang²  Zida Huang²  Wenbo Li²  Chaofan Zhang²  Yuanqing Cai²  Xinyu Fang²  Zhenpeng Guan³ 

[1] Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China;Department of Orthopaedic Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China;Department of Orthopedic Surgery, Peking University Shougang Hospital, Beijing, China;

关键词: Pretreatment methods;    Tissues;    Microbial recovery;    Periprosthetic joint infection;    Periprosthetic tissues;   

DOI  :  10.1186/s12891-021-04071-5

来源: Springer

【 摘 要 】

BackgroundThis study aimed to evaluate the effects of different pretreatment methods on the microbial yield from infectious tissues.MethodsStrains of Staphylococcus aureus (SA), Escherichia coli (EC) and Candida albicans (CA) were used to construct single-surface, full-surface, and internal infection models in sterile pork tissue. Manual milling (MM), mechanical homogenization (MH), sonificated (SF), dithiothreitol (DTT), and direct culture (DC) were used to pretreat these tissues, the microbial yield from different pretreatment methods were recorded and compared. Moreover, periprosthetic tissues collected intraoperatively from periprosthetic joint infection (PJI) patients were used as a verification.ResultsThe study showed that the microbial yield from MH pretreatment was significantly higher than that of MM (P < 0.01) and SF pretreatment method (P < 0.01). Furthermore, in the internal infection model, the microbial yield from MH group was also significantly higher than that of SF (P < 0.01), DTT (P < 0.01), and DC group (P < 0.01). Moreover, the number of bacterial colonies obtained from periprosthetic tissues pretreated by MH was significantly higher than pretreated by other pretreatment methods (P = 0.004).ConclusionsThe effects of MH and DTT in microbial yield were significantly higher than that of DC, SF and MM, and these methods can be used to process multiple tissue samples at the same time, which might further improve the diagnostic sensitivity of infectious disease.

【 授权许可】

CC BY