Study of growth, metabolism, and morphology of Akkermansia muciniphila with an in vitro advanced bionic intestinal reactor

BMC Microbiology

Min-jie Gao¹  Guoao Hu¹  Xiaobei Zhan¹  Zhitao Li¹  Yun Jiang¹  Zhenglong Sun²  Li Zhu³ 

[1] Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China;Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, 215163, Suzhou, China;Wuxi Galaxy Biotech Co. Ltd., 214125, Wuxi, China;

关键词: Akkermansia muciniphila;    Anaerobic fermentation;    Bioreactor;    Short-chain fatty acids;   

DOI  :  10.1186/s12866-021-02111-7

来源: Springer

【 摘 要 】

BackgroundAs a kind of potential probiotic, Akkermansia muciniphila abundance in human body is directly causally related to obesity, diabetes, inflammation and abnormal metabolism. In this study, A. muciniphila dynamic cultures using five different media were implemented in an in vitro bionic intestinal reactor for the first time instead of the traditional static culture using brain heart infusion broth (BHI) or BHI + porcine mucin (BPM).ResultsThe biomass under dynamic culture using BPM reached 1.92 g/L, which improved 44.36% compared with the value under static culture using BPM. The biomass under dynamic culture using human mucin (HM) further increased to the highest level of 2.89 g/L. Under dynamic culture using porcine mucin (PM) and HM, the main metabolites were short-chain fatty acids (acetic acid and butyric acid), while using other media, a considerable amount of branched-chain fatty acids (isobutyric and isovaleric acids) were produced. Under dynamic culture Using HM, the cell diameters reached 999 nm, and the outer membrane protein concentration reached the highest level of 26.26 μg/mg.ConclusionsThis study provided a preliminary theoretical basis for the development of A. muciniphila as the next generation probiotic.

【 授权许可】

CC BY