LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer

BMC Pulmonary Medicine

Lei Xian¹  Feihai Liang¹  Wenzhou Liu¹  Guangyu Yang¹ 

[1] Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital of Guangxi Medical University, No.166 Da Xuedong Road, 530007, Nanning City, Guangxi Province, People’s Republic of China;

关键词: Small cell lung cancer;    LINC01116;    miR-93-5p;    Survival;    STAT3;   

DOI  :  10.1186/s12890-020-01369-3

来源: Springer

【 摘 要 】

BackgroundLINC01116 is a recently identified oncogenic lncRNA in glioma. Differential expression analysis using the public gene expression analysis tool GEPIA revealed the upregulation of LINC01116 in lung cancer. We studied the functions of LINC01116 in small cell lung cancer (SCLC).MethodsThe expression of LINC01116 in several types of cancer tissue and the paired non-tumor tissues was evaluated by GEPIA. The effects of the overexpression of LINC01116 and miR-93-5p on the expression of STAT3 were evaluated. The effects of the overexpression of LINC01116, miR-93-5p and STAT3 on SHP-77 cell behaviors were evaluated by Transwell assays.ResultsLINC01116 was highly expressed in SCLC and predicted poor survival. In SCLC tissues, the expression of LINC01116 was positively correlated with STAT3. Bioinformatics analysis revealed that miR-93-5p may target LINC01116. Overexpression of LINC01116 increased STAT3 but did not affect the expression of miR-93-5p. Transwell assay showed that LINC01116 and STAT3 increased cell invasion and migration rates. MiR-93-5p played an suppressed cell behaviors and suppressed the role of LINC01116.ConclusionTherefore, LINC01116 might upregulate STA3 by sponging miR-93-5p, thereby promoting cell invasion and migration in SCLC.

【 授权许可】

CC BY