Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1+/−−deficient mouse

Stem Cell Research & Therapy

Walter C. Low¹  Aleta R. Steevens²  Matthew W. Griesbach³  Yun You⁴  James R. Dutton⁵  Peter A. Santi⁶ 

[1] Department of Neurosurgery, University of Minnesota, Minneapolis, MN, USA;Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA;Department of Ophthalmology, University of Minnesota, Minneapolis, MN, USA;Department of Neurosurgery, University of Minnesota, Minneapolis, MN, USA;Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA;Department of Otolaryngology, University of Minnesota, Minneapolis, MN, USA;Mouse Genetics Laboratory, University of Minnesota, Minneapolis, MN, USA;Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA;Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA;Department of Otolaryngology, University of Minnesota, Minneapolis, MN, USA;

关键词: Inner ear;    Spiral ganglion neurons;    Neurogenin1;    Blastocyst complementation;    Stem cells;    Regenerative medicine;   

DOI  :  10.1186/s13287-021-02184-1

来源: Springer

【 摘 要 】

This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1+/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.

【 授权许可】

CC BY