A performance comparison of the fully automated urine particle analyzer UF-5000 with UF-1000i and Gram staining in predicting bacterial growth patterns in women with uncomplicated urinary tract infections

BMC Urology

Chun-Chun Yang¹  Yi-Sheng Chen²  Stephen Shei-Dei Yang²  Shang-Jen Chang² 

[1] Division of General Laboratory, Taipei Tzu Chi Hospital, New Taipei, Taiwan;Division of Urology, Department of Surgery, Taipei Tzu Chi Hospital, New Taipei, Taiwan;School of Medicine, Tzu Chi University, Hualien, Taiwan;

关键词: Urinary tract infection;    Diagnosis;    Laser flow cytometry;    Fully automated urine particle analyzer;   

DOI  :  10.1186/s12894-021-00791-x

来源: Springer

【 摘 要 】

BackgroundThe aim of this study was to compare the performance of the new flow cytometer UF-5000 with the UF-1000i and Gram staining for determining bacterial patterns in urine samples.MethodsWomen who attended our clinic with symptoms suggestive of urinary tract infection were enrolled in the study. Mid-stream urine samples were collected for gram staining, urine analysis and urine cultures. Bacterial patterns were classified using the UF-1000i (none, cocci bacteria or rods/mixed growth), the UF-5000 (none, cocci, rods or mixed growth) and Gram staining.ResultsAmong the 102 included samples, there were 10 g-positive cocci, 2 g-positive bacilli, 66 g-negative rods, and 24 mixed growth. The sensitivity/specificity of the UF-1000i was 81.8/91.1% for gram-negative rods and 23.5/96.9% for cocci/mixed. The sensitivity/specificity of the UF-5000 was 80.0/88.2% for gram negative rods and 70.0/86.5% for gram-positive cocci.ConclusionsThe UF-5000 demonstrated good sensitivity and specificity for Gram-negative bacilli and demonstrated an improved sensitivity for detecting Gram-positive cocci compared with the UF-1000i.

【 授权许可】

CC BY