Molecular detection and genotyping of bovine viral diarrhea virus in Western China

BMC Veterinary Research

Xiaomin Zhao¹  Yanping Qi¹  Lingling Chang¹  Dan Liu¹  Dewen Tong¹  Qian Du¹ 

[1] College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China;

关键词: BVDV;    RT-PCR;    Genotype;    Bovine;    Western China;   

DOI  :  10.1186/s12917-021-02747-7

来源: Springer

【 摘 要 】

BackgroundBovine viral diarrhea virus (BVDV) is an important global viral pathogen of cattle and other ruminants. To survey the infection rate and genetic diversity of BVDV in western China, a total of 1234 serum samples from 17 herds of dairy cattle, beef cattle and yak in 4 provinces were collected in 2019.ResultsAll the 1234 serum samples were screened individually for BVDV by RT-PCR. Our results demonstrated that the average positive rate of BVDV was 7.2% (89/1234) in animals and 82.4% (14/17) in herds. Thirteen BVDV strains were isolated from RT-PCR positive clinical samples and they were all NCP biotype. BVDV-1a and 1c subgenotypes were identified from 22 selected virus isolates in 14 BVDV-positive herds. These results confirmed that BVDV-1a and BVDV-1c were circulating in western China, similar to the BVDV epidemics in cattle in other regions of China.ConclusionsThis study provides data for monitoring and vaccination strategies of BVDV in western China.

【 授权许可】

CC BY