The role of microglia membrane potential in chemotaxis

Journal of Neuroinflammation

Thomas G. Oertner¹  Christian Schulze¹  Marie-Luise Brehme¹  Laura Laprell¹ 

[1] Institute for Synaptic Physiology, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf, Falkenried 94, 20251, Hamburg, Germany;

关键词: Microglia;    Sterile inflammation;    Two-photon microscopy;    Optogenetics;    Hippocampus;    Channelrhodopsin;    Chemotaxis;    Calcium signaling;    Laser damage;    Chemotaxis;   

DOI  :  10.1186/s12974-020-02048-0

来源: Springer

【 摘 要 】

Microglia react to danger signals by rapid and targeted extension of cellular processes towards the source of the signal. This positive chemotactic response is accompanied by a hyperpolarization of the microglia membrane. Here, we show that optogenetic depolarization of microglia has little effect on baseline motility, but significantly slows down the chemotactic response. Reducing the extracellular Ca2+ concentration mimics the effect of optogenetic depolarization. As the membrane potential sets the driving force for Ca2+ entry, hyperpolarization is an integral part of rapid stimulus-response coupling in microglia. Compared to typical excitable cells such as neurons, the sign of the activating response is inverted in microglia, leading to inhibition by depolarizing channelrhodopsins.

【 授权许可】

CC BY